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Effect of loading and vitrification solutions on survival of cryopreserved oil palm polyembryoids

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Abstract

The present study evaluates the effect of six loading solutions and five vitrification solutions (VS) and their time of exposure on the survival of oil palm (Elaeis guineensis) polyembryoids in liquid nitrogen (LN). In vitro grown polyembryoids of oil palm were successfully cryopreserved by vitrification with 45% survival. Individual polyembryoids, isolated from 2-month old culture, were precultured in liquid Murashige and Skoog medium supplemented with 0.5 M sucrose for 12 h and treated with a mixture of 10% (w/v) dimethyl sulphoxide (DMSO) plus 0.7 M sucrose for 30 min. Polyembryoids were then subjected to plant vitrification solution-2 (PVS2) (30% (w/v) glycerol plus 15% (w/v) EG plus 15% (w/v) DMSO plus 0.4 M sucrose) exposure for 5 min at 26 ± 2°C and subsequently plunged into LN. Thawed polyembryoids resumed growth within 8 days of culture and shoot development was recorded at 25 days of growth. Scanning electron micrograph revealed that successful regeneration of cryopreserved polyembryoids was due to stabilization of cellular integrity through optimum VS exposure.

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Fig. 1

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Abbreviations

LN:

Liquid nitrogen

LS:

Loading solution

MS:

Murashige and Skoog (1962)

SEM:

Scanning electron microscope

VS:

Vitrification solution

MC:

Moisture content

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Acknowledgments

The authors are grateful to Department of Crop Science, Faculty of Agriculture, Universiti Putra, Malaysia for the facilities and Yayasan Felda, Malaysia for the financial support through research grant.

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Authors and Affiliations

  1. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Periasamy Suranthran, Saikat Gantait & Uma Rani Sinniah

  2. School of Biological Sciences, Universiti Sains Malaysia, Minden Heights, 11800, Penang, Malaysia

    Sreeramanan Subramaniam

  3. Felda Biotechnology Center (FBC), Bandar Enstek, Sepang, Selangor, Malaysia

    Sharifah Shahrul Rabiah Syed Alwee & Siti Habsah Roowi

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  1. Periasamy Suranthran
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  2. Saikat Gantait
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  3. Uma Rani Sinniah
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  4. Sreeramanan Subramaniam
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  5. Sharifah Shahrul Rabiah Syed Alwee
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  6. Siti Habsah Roowi
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Correspondence to Uma Rani Sinniah.

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Suranthran, P., Gantait, S., Sinniah, U.R. et al. Effect of loading and vitrification solutions on survival of cryopreserved oil palm polyembryoids. Plant Growth Regul 66, 101–109 (2012). https://doi.org/10.1007/s10725-011-9633-7

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  • DOI: https://doi.org/10.1007/s10725-011-9633-7

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