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Involvement of abscisic acid and hydrogen peroxide in regulating the activities of antioxidant enzymes in leaves of rice seedlings under magnesium deficiency

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Abstract

Magnesium (Mg) deficiency in plants is a widespread problem, affecting productivity and quality in agriculture. The mechanism of Mg deficiency inducing antioxidant enzyme activities has not been elucidated in rice. We examined the relationship among abscisic acid (ABA), H2O2, and antioxidant enzymes in the leaves of rice seedlings grown under conditions of Mg deficiency. The expression of OsRab16A, an ABA responsive gene, was used to determine the content of ABA. Mg deficiency resulted in increased ABA content in leaves of rice seedlings. The production of H2O2 was examined by 3,3-diaminobenzidine staining and a colorimetric method. Mg deficiency also induced H2O2 production in leaves, which was blocked by dipehnyleneiodonium chloride (DPI), an NADPH oxidase inhibitor. Tungstate (Tu), an ABA biosynthesis inhibitor, was effective in reducing Mg deficiency-increased ABA content, as well as Mg deficiency-induced H2O2 production. Both Tu and DPI were effective in reducing Mg deficiency-induced activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and catalase in the leaves. Mg deficiency-induced ABA accumulation may trigger increased production of H2O2, which may involve plasma-membrane NADPH oxidase, and, in turn, up-regulates the activities of antioxidant enzymes in leaves of rice seedlings.

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Abbreviations

ABA:

Abscisic acid

APX:

Ascorbate peroxidase

AsA:

Ascorbic acid

CAT:

Catalase

DAB:

3,3-Diaminobenzidine

DPI:

Diphenyleneiodonium chloride

FM:

Fresh mass

GR:

Glutathione reductase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Tu:

Sodium tungstate

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Acknowledgments

This work was supported by a research grant from the National Science Council of the Republic of China.

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Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, ROC

    Yun-Yang Chao, Ting-Shao Chou & Ching Huei Kao

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  1. Yun-Yang Chao
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  2. Ting-Shao Chou
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  3. Ching Huei Kao
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Correspondence to Ching Huei Kao.

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Chao, YY., Chou, TS. & Kao, C.H. Involvement of abscisic acid and hydrogen peroxide in regulating the activities of antioxidant enzymes in leaves of rice seedlings under magnesium deficiency. Plant Growth Regul 66, 1–8 (2012). https://doi.org/10.1007/s10725-011-9623-9

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  • DOI: https://doi.org/10.1007/s10725-011-9623-9

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