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Combined effect of salicylic acid and salinity on some antioxidant activities, oxidative stress and metabolite accumulation in Phaseolus vulgaris

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Abstract

It has been shown that salicylic acid (SA) acts as an endogenous signal molecule responsible for inducing abiotic stress tolerance in plants. The effect of SA and sodium chloride (NaCl) on growth, metabolite accumulation, oxidative stress and enzymatic and non-enzymatic antioxidant responses on common bean plants (Phaseolus vulgaris, cv. F-15) was studied. Results revealed that either SA or NaCl decrease, shoot, root and total plant dry weights. SA treatments decreased the contents of proline, and reduced forms of ascorbate and glutathione, however, the content of soluble sugars (TSS), thiobarbituric acid-reactive substances (TBARs) and oxidized ascorbate remained unaffected. On the other hand, salinity significantly reduced the levels of endogenous SA but increased the content of proline, soluble sugars, TBARs, ascorbate and glutathione, as well as all increasing the levels of antioxidant enzyme activities assayed, except CAT. The application of SA improved the response of common bean plants to salinity by increasing plant dry weight and decreasing the content of organic solutes (proline and TSS) and damage to the membrane (TBARs). Moreover, SA application under saline conditions decreased the levels of antioxidant enzyme activities POX, APX and MDHAR which could indicate successful acclimatization of these plants to saline conditions.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

DHA:

Dehydroascorbate

DHAR:

Dehydroascorbate reductase

GR:

Glutathione reductase

POX:

Guaiacol peroxidase

MDHAR:

Monodehydroascorbate reductase

GSSG:

Oxidized glutathione

ROS:

Reactive oxygen species

AsA:

Reduced ascorbate

GSH:

Reduced glutathione

SA:

Salicylic acid

TBARs:

Thiobarbituric acid-reactive substances

TCA:

Trichloroacetic acid

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Acknowledgments

This work was financially supported by the Spanish Ministry of Science and Technology (AGL2006-01279/AGR). The authors are grateful Dr. Pieter Van Dillewijn for the English correction of the paper. We also thank to anonymous reviewers for making valuable suggestions to earlier drafts of this study.

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Authors and Affiliations

  1. Departamento de Fisiología Vegetal, Facultad de Ciencias, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain

    Francisco Palma, Carmen Lluch, Carmen Iribarne & Noel A. Tejera García

  2. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain

    Jose M. García-Garrido

Authors
  1. Francisco Palma
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  2. Carmen Lluch
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  4. Jose M. García-Garrido
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  5. Noel A. Tejera García
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Correspondence to Noel A. Tejera García.

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Palma, F., Lluch, C., Iribarne, C. et al. Combined effect of salicylic acid and salinity on some antioxidant activities, oxidative stress and metabolite accumulation in Phaseolus vulgaris . Plant Growth Regul 58, 307–316 (2009). https://doi.org/10.1007/s10725-009-9380-1

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