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Characterization of the complete mitochondrial genome of flower-breeding Drosophila incompta (Diptera, Drosophilidae)

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Abstract

Drosophila incompta belongs to the flavopilosa group of Drosophila, and has a restricted ecology, being adapted to flowers of Cestrum as feeding and oviposition sites. We sequenced, assembled, and characterized the complete mitochondrial genome (mtDNA) of D. incompta. In addition, we performed phylogenomic and polymorphism analyses to assess evolutionary diversification of this species. Our results suggest that this genome is syntenic with the other published mtDNA of Drosophila. This molecule contains 15,641 bp and encompasses two rRNA, 22 tRNA and 13 protein-coding genes. Regarding nucleotide composition, we found a high A−T bias (76.6 %). The recovered phylogenies indicate D. incompta in the virilis–repleta radiation, as sister to the virilis or repleta groups. The most interesting result is the high degree of polymorphism found throughout the D. incompta mitogenome, revealing pronounced intrapopulational variation. Furthermore, intraspecific nucleotide diversity levels varied between different regions of the genome, thus allowing the use of different mitochondrial molecular markers for analysis of population structure of this species.

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Acknowledgments

This study was supported by research grants and fellowships from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), PRONEX-FAPERGS (10/0028-7) and FAPERGS (11/0938-0).

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Authors and Affiliations

  1. Programa de Pós Graduação em Biodiversidade Animal, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil

    F. C. De Ré, G. L. Wallau, L. J. Robe & E. L. S. Loreto

  2. Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal do Pampa, São Gabriel, Rio Grande do Sul, Brazil

    G. L. Wallau

  3. Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil

    L. J. Robe

  4. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil

    E. L. S. Loreto

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  1. F. C. De Ré
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  4. E. L. S. Loreto
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Correspondence to E. L. S. Loreto.

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De Ré, F.C., Wallau, G.L., Robe, L.J. et al. Characterization of the complete mitochondrial genome of flower-breeding Drosophila incompta (Diptera, Drosophilidae). Genetica 142, 525–535 (2014). https://doi.org/10.1007/s10709-014-9799-9

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