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Validation and diagnostic marker development for a genetic region associated with wheat yellow mosaic virus resistance

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Abstract

Wheat yellow mosaic disease (WYM) became more serious in China in the recent years. Cultivation of resistant varieties is the major strategy for disease control. To identify WYM resistant genes in Chinese wheat cultivar ‘Yining Xiaomai’, a population of 106 recombinant inbred lines (RIL) from a cross between Yining Xiaomai and Zhen 9523 (WYM-susceptible) was generated. Phenotypic data of WYM resistance of the RILs were collected over three successive years in a naturally infected field nursery. A linkage map consisting of 29 genetic linkage groups involving 144 SSR or EST markers was constructed, using the JoinMap 4.0 software. By composite interval mapping using IciMapping 4.0, a stable major QTL associated with WYM resistance, designated QYm.nau-2D, was mapped to chromosome 2D which explained up to 93.2 % of the phenotypic variance. More linkage markers including 11 SNP and 11 STS markers were developed to saturate the QYm.nau-2D region. The presence of WYM resistance was further validated using a resistant RIL derived F2 population consisting 318 lines. A high resolution map at QYm.nau-2D region was re-constructed which established a basis for map-based cloning of the resistant gene. Four markers were validated to be diagnostic markers to discriminate 195 breeding lines with known pedigree. These markers could be used in marker-assisted selection to breeding WYM resistance.

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References

  • Barbosa MM, Goulart LR, Prestes AM, Juliatti FC (2001) Genetic control of resistance to soilborne wheat mosaic virus in Brazilian cultivars of Triticum aestivum L. Thell Euphytica 122:417–422

    Article  Google Scholar 

  • Bass C, Hendley R, Adams MJ, Hammond-Kosack KE, Kanyuka K (2006) The Sbm1 locus conferring resistance to soil-borne cereal mosaic virus maps to a gene-rich region on 5DL in wheat. Genome 49:1140–1148

    Article  CAS  PubMed  Google Scholar 

  • Bassam BJ, Gresshoff PM (2007) Silver staining DNA in polyacrylamide gels. Nat Protoc 2:2649–2654

    Article  CAS  PubMed  Google Scholar 

  • Chen JP (2005) Progress and prospects of studies on Polymyxa graminis and its transmitted cereal viruses in China. Prog Nat Sci 15:481–490

    Article  CAS  Google Scholar 

  • Chen JP, Ruan YL, Dong MJ (1989) Study on the pathogen of a wheat soil-borne virus disease in China. Virol Sin 2:176–181

    Google Scholar 

  • Clover G, Henry C (1999) Detection and discrimination of wheat spindle streak mosaic virus and wheat yellow mosaic virus using multiplex RT-PCR. Eur J Plant Pathol 105:891–896

    Article  CAS  Google Scholar 

  • Han CG, Li DW, Xing YM, Zhu K, Tian ZF, Cai ZN, Yu JL, Liu Y (2000) Wheat yellow mosaic virus widely occurring in wheat (Triticum aestivum) in China. Plant Dis 84:627–630

    Article  Google Scholar 

  • Hariri D, Delaunay T, Gomes L, Filleur S, Plovie C, Lapierre H (1996) Comparison and differentiation of wheat yellow mosaic virus (WYMV), wheat spindle streak mosaic virus (WSSMV) and barley yellow mosaic virus (BaYMV) isolates using WYMV monoclonal antibodies. Eur J Plant Pathol 102:283–292

    Article  Google Scholar 

  • Inouye T (1969) Viral pathogen of the wheat yellow mosaic disease. Nogaku Kenkyu 53:61–68

    Google Scholar 

  • Khan AA, Bergstrom GC, Nelson JC, Sorrells ME (2000) Identification of RFLP markers for resistance to wheat spindle streak mosaic bymovirus (WSSMV) disease. Genome 43:477–482

    Article  CAS  PubMed  Google Scholar 

  • Kojima H, Nishio Z, Kobayashi F, Saito M, Sasaya T, Kiribuchi-Otobe C, Seki M, Oda S, Nakamura T (2015) Identification and validation of a quantitative trait locus associated with wheat yellow mosaic virus pathotype I resistance in a Japanese wheat variety. Plant Breed 134:373–378

    Article  CAS  Google Scholar 

  • Kuhne T (2009) Soil-borne viruses affecting cereals-known for long but still a threat. Virus Res 141:174–183

    Article  PubMed  Google Scholar 

  • Lei J, Chen J, Chen J, Zheng T, Cheng Y (1998) Identification of Chinese fungal-transmitted filamentous wheat mosaic virus by RT-PCR and SSCP. Zhongguo Bing Du Xue 13:89–96

    CAS  Google Scholar 

  • Liu WH, Nie H, Wang SB, Li X, He ZT, Han CG, Wang JR, Chen XL, Li LH, Yu JL (2005) Mapping a resistance gene in wheat cultivar Yangfu 9311 to yellow mosaic virus, using microsatellite markers. Theor Appl Genet 111:651–657

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Wu H, Chen H, Liu Y, He J, Kang H, Sun Z, Pan G, Wang Q, Hu J, Zhou F, Zhou K, Zheng X, Ren Y, Chen L, Wang Y, Zhao Z, Lin Q, Wu F, Zhang X, Guo X, Cheng X, Jiang L, Wu C, Wang H, Wan J (2014) A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice. Nat Biotechnol 33:301–305

    Article  CAS  PubMed  Google Scholar 

  • Lommel S, Willis W, Kendall T (1986) Identification of wheat spindle streak mosaic virus and its role in a new disease of winter wheat in Kansas. Plant Dis 70:964–968

    Article  Google Scholar 

  • Lu XY, Kashiwazaki S, Tamura M, Namba S (1998) The 3’ terminal sequence of RNA1 of wheat spindle streak mosaic virus Canadian isolate (WSSMV-C). Eur J Plant Pathol 104:765–768

    Article  Google Scholar 

  • Namba S, Kashiwazaki S, Lu X, Tamura M, Tsuchizaki T (1998) Complete nucleotide sequence of wheat yellow mosaic Bymovirus genomic RNAs. Arch Virol 143:631–643

    Article  CAS  PubMed  Google Scholar 

  • Nishio Z, Kojima H, Hayata A, Iriki N, Tabiki T, Ito M, Yamauchi H, Murray TD (2010) Mapping a gene conferring resistance to Wheat yellow mosaic virus in European winter wheat cultivar ‘Ibis’ (Triticum aestivum L.). Euphytica 176:223–229

    Article  CAS  Google Scholar 

  • Ostrom RS, Naugle JE, Hase M, Gregorian C, Swaney JS, Insel PA, Brunton LL, Meszaros JG (2003) Angiotensin II enhances adenylyl cyclase signaling via Ca2 +/calmodulin. Gq-Gs cross-talk regulates collagen production in cardiac fibroblasts. J Biol Chem 278:24461–24468

    Article  CAS  PubMed  Google Scholar 

  • Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107:1235–1242

    Article  CAS  PubMed  Google Scholar 

  • Pestsova E, Salina E, Borner A, Korzun V, Maystrenko OI, Roder MS (2000) Microsatellites confirm the authenticity of inter-varietal chromosome substitution lines of wheat (Triticum aestivum L.). Theor Appl Genet 101:95–99

    Article  CAS  Google Scholar 

  • Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD, Akhunov ED, Dvorak J, Linkiewicz AM, Ratnasiri A, Dubcovsky J, Bermudez-Kandianis CE, Greene RA, Kantety R, La Rota CM, Munkvold JD, Sorrells SF, Sorrells ME, Dilbirligi M, Sidhu D, Erayman M, Randhawa HS, Sandhu D, Bondareva SN, Gill KS, Mahmoud AA, Ma XF, Miftahudin, Gustafson JP, Conley EJ, Nduati V, Gonzalez-Hernandez JL, Anderson JA, Peng JH, Lapitan NLV, Hossain KG, Kalavacharla V, Kianian SF, Pathan MS, Zhang DS, Nguyen HT, Choi DW, Fenton RD, Close TJ, McGuire PE, Qualset CO, Gill BS (2004) A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics 168:701–712

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rubies-Autonell C, Vallega V (1987) Observations on a mixed soil-borne Wheat mosaic virus and wheat spindle streak mosaic virus infection in durum wheat (Triticum durum Desf.). J Phytopathol 119:111–121

    Article  Google Scholar 

  • Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290

    Article  CAS  Google Scholar 

  • Silva LDCE, Wang S, Zeng ZB (2012) Composite interval mapping and multiple interval mapping: procedures and guidelines for using Windows QTL Cartographer. Methods Mol Biol 871:75–119

    Article  CAS  Google Scholar 

  • Slykhuis J, Polak Z (1969) Verification of wheat spindle streak mosaic virus as a cause of mosaic of wheat in Ontario. Plant Dis Surv 49:108–111

    Google Scholar 

  • Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560

    Article  CAS  PubMed  Google Scholar 

  • Suzuki T, Murai MN, Hayashi T, Nasuda S, Yoshimura Y, Komatsuda T (2015) Resistance to wheat yellow mosaic virus in Madsen wheat is controlled by two major complementary QTLs. Theor Appl Genet 128:1569–1578

    Article  CAS  PubMed  Google Scholar 

  • Van Ooijen JW (2011) Multipoint maximum likelihood mapping in a full-sib family of an outbreeding species. Genet Res 93:343–349

    Article  Google Scholar 

  • Wang HG, George MS, Kim J, Wang C, Pitt GS (2007) Ca2+/calmodulin regulates trafficking of Ca(V)1.2 Ca2 + channels in cultured hippocampal neurons. J Neurosci 27:9086–9093

    Article  CAS  PubMed  Google Scholar 

  • Wu JR, Yu MZ, Xu JH, Du J, Ji F, Dong F, Li XH, Shi JR (2014) Impact of transgenic wheat with wheat yellow mosaic virus resistance on microbial community diversity and enzyme activity in rhizosphere soil. Plos One 9:e98394

    Article  PubMed  PubMed Central  Google Scholar 

  • Xue SL, Zhang ZZ, Lin F, Kong ZX, Cao Y, Li CJ, Yi HY, Mei MF, Zhu HL, Wu J, Xu HB, Zhao DM, Tian DG, Zhang CQ, Ma Z (2008) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181–189

    Article  CAS  PubMed  Google Scholar 

  • Zhang QP, Wang XE, Wang YN, Zhao Y, Wang HY, Wang SL, Chen PD (2005) Inheritance analysis and molecular marker selection of genes for wheat spindle streak mosaic disease resistance. Acta Genetica Sinica 32(7):733–737

    CAS  PubMed  Google Scholar 

  • Zhu X, Wang H, Guo J, Wu Z, Cao A, Bie T, Nie M, You FM, Cheng Z, Xiao J, Liu Y, Cheng S, Chen P, Wang X (2012) Mapping and validation of quantitative trait loci associated with wheat yellow mosaic Bymovirus resistance in bread wheat. Theor Appl Genet 124:177–188

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The research was supported by National Natural Science Foundation of China (Grant No. 31171541), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Technology Support Program of Jiangsu Province (Grant No. BE2015352), the Program of Introducing Talents of Discipline to Universities (Grant No. B08025), Project Development of Seed Industry for Shanghai Agricultural World (2012), Fundamental Research Funds for the Central Universities (Grant No. KYZ201202), high-level talents in six industries of Jiangsu Province and the Project No. 7 from Science and High-Tech Based Major Program of Agriculture Committee of Shanghai Municipal Administration (Grant No. 20127). We are grateful to Dr. Robert McIntosh for his useful comments about the research and the critical reading and revise of the manuscript.

Authors’ contributions

XJ, WXE, WHY and CXH designed experimental plan. GJ, CXL, WZZ and XZT performed experiments. NMJ, BTD, ZXB evaluated WYM resistance. ZTT, LMC and FY designed the SNP markers. XJ, WXE, GJ and XZT wrote the manuscript. All authors have read and approved the final manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu Province, China

    Jin Xiao, Xueluan Chen, Zhitian Xu, Jiao Guo, Zhenzhen Wu, Haiyan Wang, Xiaobiao Zhu, Mingjuan Nie & Xiue Wang

  2. Department of Wheat Breeding, Agricultural Sciences Institute of the Lixiahe District, Yangzhou, 225007, Jiangsu Province, China

    Tongde Bie & Shunhe Cheng

  3. Department of Plant Sciences, University of California, Davis, CA, 95616, USA

    TingTing Zhu & Mingcheng Luo

  4. Agriculture and Agri-Food Canada, 101 Route 100, Morden, MB, R6M 1Y5, Canada

    Frank M. You

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  1. Jin Xiao
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  2. Xueluan Chen
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  3. Zhitian Xu
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  14. Xiue Wang
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Correspondence to Xiue Wang.

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10681_2016_1731_MOESM1_ESM.pdf

Supplementary Fig. 1: RT-PCR detection of WSSMV by primer pairs WMVCPF/WMVCPR (a) and Wp1(+)/Wp2(−) (b) and of WSSMV by (W2(+)/W9(−) (c) and WSSMVF/Oligo-d(T)Not1) (d), using the tubulin gene (e) for normalization of the cDNA concentration

Supplementary Fig. 2: Linkage maps for the Yining Xiaomai × Zhen 9523 F2:8 RIL population. Numbers on the left are genetic distances in centiMorgans (cM). Names of the molecular markers are shown on the right

Supplementary Fig. 3: Chromosome bin assignment of marker Xcfd267 using Chinese Spring nulli-tetrasomic and deletion lines (PDF 953 kb)

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Xiao, J., Chen, X., Xu, Z. et al. Validation and diagnostic marker development for a genetic region associated with wheat yellow mosaic virus resistance. Euphytica 211, 91–101 (2016). https://doi.org/10.1007/s10681-016-1731-1

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