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The self-incompatible RNase S-alleles of Brazilian apple cultivars

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Abstract

Apple plants are self-incompatible because a genetic mechanism allows the female reproductive organ to recognize and reject self-pollen or pollen from genetic related individuals and allows non-self pollen to effect fertilization. Thus, there are implications to both breeding strategies and orchard management for fruit production. The purpose of this study was to identify and to characterize the S-RNase alleles of the gametophytic incompatibility among apple cultivars developed in Brazil, seeking to give support for choosing right combinations of parent in the apple breeding programs. It also sought to identify correct combinations of scion/pollinator cultivars of commercial apple orchards. A total of 16 specific S-RNase alleles primers were tested against DNA extracted from 12 Brazilian cultivars and their parents. The molecular analysis confronted to the reference cultivars, showed that the cultivars Daiane, Imperatriz and Princesa have the same incompatibility S3 and S5 alleles, while ‘Lisgala’ showed the alleles S2 and S5; ‘Suprema’, S1 and S9; ‘Catarina’, S1 and S19; ‘Joaquina’ and ‘Fred Hough’, S5 and S19; ‘Baronesa’, S3 and S9; ‘Duquesa’, S2 and S3. For ‘Primícia’ and ‘Condessa’ it was only possible to identify one of the S-alleles, namely S24 and S2, respectively, with the second allele remaining to be identified. Progeny test indicated the Mendelian inheritance for RNase alleles. Results of this study will be helpful to judiciously choose parents in apple breeding programs to improve compatibility.

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References

  • Broothaerts W (2003) New findings in apple S-genotype analysis resolve previous confusion and request the re-numbering of some S-alleles. Theor Appl Genet 106:703–714

    PubMed  CAS  Google Scholar 

  • Broothaerts W (2004) Update on and review of the incompatibility (S-) genotypes of apple cultivars. HortScience 39:943–947

    CAS  Google Scholar 

  • Broothaerts W, Janssens GA, Proost P, Brokaert WF (1995) cDNA cloning and molecular analysis of two self-incompatibility alleles from apple. Plant Mol Biol 27:499–511

    Article  PubMed  CAS  Google Scholar 

  • Carrera L, Sanzol J, Herrero M, Hormaza JI (2009) Genomic characterization of self-incompatibility ribonucleases (S-RNases) in loquat (Eriobotrya japonica Lindl.) (Rosaceae, Pyrinae). Mol Breeding 23:539–551

    Article  CAS  Google Scholar 

  • de Nettancourt D (1977) Incompatibility in angiosperms. In: Frankel R, Gal GAE, Linskens HF (eds) Monographs on theoretical and applied genetics. Springer, Berlin, pp 28–57

    Google Scholar 

  • Doyle JJ, Doyle JL (1987) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Dressen RSG, Vanholme BTM, Luyten K, Wynsberghe LV, Fazio G, Roldán-Ruiz I, Keulemans J (2010) Analysis of Malus S-Rnase gene diversity based on a comparative study of old and modern apple cultivars and European wild apple. Mol Breeding 26:693–709

    Article  Google Scholar 

  • Ishimizu T, Inoue K, Shimonaka M, Saito T, Terai O, Norioka S (1999) PCR-based method for identifying the S-genotypes of Japanese pear cultivars. Theor Appl Genet 98:961–967

    Article  CAS  Google Scholar 

  • Janssens GA, Goderis IJ, Broekaert WF, Broothaerts W (1995) A molecular method for S-allele identification in apple based on allele-specific PCR. Theor Appl Genet 91:691–698

    Article  CAS  Google Scholar 

  • Kim H, Kakui H, Kotoda N, Hirata Y, Koba T, Sassa H (2009) Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S-haplotypes of apple (Malus × domestica Borkh). Mol Breeding 23:463–472

    Article  CAS  Google Scholar 

  • Kitahara KM, Matsumoto S (2002) Sequence of the S10 cDA from ‘McIntosh’ apple and a PCR-digestion identification method. HosrtScience 37:187–190

    CAS  Google Scholar 

  • Kitahara K, Soejima J, Komatsu H, Fukui H, Matsumoto S (2000) Complete sequences of the S-genes ‘Sd’ and ‘Sh-RNase’ cDNA in apple. HortScience 35:712–715

    CAS  Google Scholar 

  • Kobel F, Steinegger P, Anliker J (1939) Weitere Untersuchungen uber die Befruchtungsverhaltnisse der Apfel- und Birnsorten. Landw Jahrb Schweiz 53:160–191

    Google Scholar 

  • Manganaris AG, Alston FH (1987) Inheritance and linkage relationships of glutamate oxaloacetate transaminase isozymes in apple. 1. The gene Got-1, a maker for the S incompatibility locus. Theor Appl Genet 74:154–161

    Article  CAS  Google Scholar 

  • Matsumoto S, Kitahara K (2000) Discovery of a new selfincompatibility allele in apple. HortScience 35:1329–1332

    Google Scholar 

  • Matsumoto S, Kitara K, Komori S, Soejima J (1999) A new S-allele inapple, ‘Sg’, and its similarity to the ‘Sf’ allele from ‘Fuji’. HortScience 34:708–710

    CAS  Google Scholar 

  • Noiton D, Shelbourne CJA (1992) Quantitative genetics in an apple breeding strategy. Euphytica 60:213–219

    Google Scholar 

  • Okuno T (2000) S-RNase from Malus domestica, cultivar Starking. Delicious. GenBank submission no. AB017636

  • Ortega E, Bosković RI, Sargent DA, Tobutt KR (2006) Analysis of S-RNAse alleles of almond (Prunus dulcis): characterization of new sequences, resolution of synonyms and evidence of intragenic recombination. Mol Genet Genom 276:413–426

    Article  CAS  Google Scholar 

  • Richman AD, Broothaerts W, Kohn JR (1997) Self-incompatibility RNases from three plant families: homology or convergence? Am J Bot 84:912–917

    Article  PubMed  CAS  Google Scholar 

  • Sakurai K, Brown SK, Weeden NF (1997) Determining the self-incompatibility alleles of Japanese apple cultivars. HortScience 32:1258–1259

    Google Scholar 

  • Sakurai K, Brown SK, Weeden NF (2000) Self-incompatibility alleles of apple cultivars and advanced selections. HortScience 35:116–119

    CAS  Google Scholar 

  • Sassa H, Mase N, Hirano H, Ikehashi H (1994) Identification of self-incompatibility-related glycoproteins in styles of apple (Malus × domestica). Theor Appl Genet 89:201–205

    Article  CAS  Google Scholar 

  • Sassa H, Nishio T, Kowyama Y, Hirano H, Koba T, Ikehashi H (1996) Self-incompatibility (S) alleles of the Rosaceae encode members of a distinct class of the T2/S ribonuclease superfamily. Mol Gen Genet 250:547–557

    PubMed  CAS  Google Scholar 

  • Takayama S, Isogai A (2005) Self-incompatibility in plants. Annu Rev Plant Biol 56:467–489

    Article  PubMed  CAS  Google Scholar 

  • Van Nerum I, Geerts M, Van Haute A, Keulemans J, Broothaerts W (2001) Re-examination of the self-incompatibility genotype of apple cultivars containing putative ‘new’ S-alleles. Theor Appl Genet 103:584–591

    Article  CAS  Google Scholar 

  • Verdoodt L, Van Haute A, Goderis IJ, De Witte K, Keulemans J, Broothaerts W (1998) Use of the multi-allele self-incompatibility gene in apple to assess homozygosity in shoots obtained through haploid induction. Theor Appl Genet 96:294–300

    Article  CAS  Google Scholar 

  • Vieira J, Morales-Hojas R, Santos RAM, Vieira CP (2007) Different positively selected sites at the gametophytic self-incompatibility pistil S-RNase gene in the Solanaceae and Rosaceae. (Prunus Pyrus and Malus) J Mol Evol 65:175–185. doi:10.1007/s00239-006-0285-6

    Article  PubMed  CAS  Google Scholar 

  • Vieira J, Ferreira PG, Aguiar B, Fonseca NA, Vieira CP (2010) Evolutionary patterns at the RNase based gametophytic self-incompatibility system in two divergent Rosaceae groups (Maloideae and Prunus). BMC Evol Biol 10:200. doi:10.1186/1471-2148-10-200

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank the National Research Council (CNPq) UNISUL, and PRODETAB for a research grant. The Brazilian CNPq provided scholarships to RON and Brazilian agency CAPES to ACMD. The authors also would like to acknowledge the anonymous reviewers, which suggestions improved the quality of the manuscript.

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Authors and Affiliations

  1. Universidade do Sul de Santa Catarina, Av. José Acácio Moreira, no. 787, C.P. 370, CEP: 88704-900, Tubarão, SC, Brazil

    Celso Lopes de Albuquerque Jr.

  2. Epagri, E.E. Caçador, C.P. 591, CEP 89500-000, Caçador, SC, Brazil

    Frederico Denardi

  3. Universidade Estadual do Rio Grande do Sul, Rua Benjamin Constant, 229, 95700-000, Bento Gonçalves, RS, Brazil

    Adriana Cibele de Mesquita Dantas

  4. Programa de pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, C.P. 476, CEP 88040-900, Florianópolis, SC, Brazil

    Rubens Onofre Nodari

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  1. Celso Lopes de Albuquerque Jr.
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  2. Frederico Denardi
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  3. Adriana Cibele de Mesquita Dantas
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  4. Rubens Onofre Nodari
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Correspondence to Rubens Onofre Nodari.

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de Albuquerque, C.L., Denardi, F., de Mesquita Dantas, A.C. et al. The self-incompatible RNase S-alleles of Brazilian apple cultivars. Euphytica 181, 277–284 (2011). https://doi.org/10.1007/s10681-011-0431-0

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  • DOI: https://doi.org/10.1007/s10681-011-0431-0

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