Summary
Four zones of enzymatic activity for glutamate oxaloacetate transaminase (GOT) were found in apple tissue. A dimeric gene, GOT-1, determining the fastest migrating zone, was identified. Six alleles were found, including a near null allelle which produced detectable heterodimeric bands but not homodimeric bands. A marked deficit or absence of certain geno-types in all backcrosses and in some crosses between unrelated varieties was attributed to the close linkage (r=0.02±0.005) of GOT-1 with the incompatibility S locus. GOT-1 was also closely linked with the isocitrate dehydrogenase locus IDH-1 (0.03±0.01). Proposed incompatibility genotypes for four cultivars, and the linked GOT-1 alleles are ‘Cox’: S 1 b/S 2 d, ‘Idared’: S 3 a/S 4 c, ‘Fiesta’: S 3 a/S 2 d and ‘Kent’: S 3 a/S 1 b.
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Communicated by H.F.Linskens
The results reported in this paper are part of a PhD Thesis by the first author
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Manganaris, A.G., Alston, F.H. Inheritance and linkage relationships of glutamate oxaloacetate transaminase isoenzymes in apple. Theoret. Appl. Genetics 74, 154–161 (1987). https://doi.org/10.1007/BF00290098
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DOI: https://doi.org/10.1007/BF00290098