Abstract
In this study, stylar proteins of apple (Malus x domestica) which correlate with known intervarietal incompatibility relationships and have similar characteristics to the S-glycoproteins of Japanese pear (Pyrus serotina) were surveyed by two-dimensional gel electrophoresis (2D-PAGE). Varietal differences were detected in a group of glycoproteins having Mrs and pIs similar to those of the S-glycoproteins of Japanese pear. 2D-PAGE profiles of these glycoproteins were correlated with intervarietal incompatibility relationships. These glycoproteins reacted with antiserum raised against the S 4-glycoprotein of Japanese pear, a result suggesting that they may be the products of S-alleles in styles of apple. On the basis of the profiles of the putative S-glycoproteins, S-genotypes were proposed for each of the apple cultivars examined.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brown SK (1992) Genetics of apple. In: Janick J (ed) Plant breeding reviews vol 9. John Wiley & Sons, New York, pp 333–366
Chyi YS, Weeden NF (1984) Relative isozyme band intensities permit the identification of the 2n gamete parent of triploid apple cultivars. Hort Science 19:818–819
Damerval C, de Vienne D, Zivy M, Thiellement H (1986) Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis 7:52–54
de Nettancourt D (1977) Incompatibility in angiosperms. In: Frankel R, Gall GAE, Linskens HF (eds) Monographs in theoretical and applied genetics vol 3. Springer, Berlin Heidelberg New York
Harada, T, Matsukawa K, Sato T, Ishikawa R, Niizeki M, Saito K (1993) DNA-RAPDs detect genetic variation and paternity in Malus. Euphytica 65:87–91
Hirano H, Watanabe T (1990) Microsequencing of proteins electroblotted onto immobilizing matrices from polyacrylamide gel electrophoresis: application to an insoluble protein. Electrophoresis 11:573–580
Ishiyama M, Kitayama H, Sato T (1993) Cross-incompatibility in apple cultivars. Abstr Jpn Soc Hortic Sci Autumn Meet. p. 162 (in Japanese)
Kijimoto-Ochiai S, Katagiri YU, Ochiai H (1985) Analysis of N-linked oligosaccharide chains of glycoproteins on nitrocellulose sheets using lectin-peroxidase reagents. Anal Biochem 147:222–229
Latimer LP (1937) Selfand cross-pollination in the McIntosh apple and some of its hybrids. Proc Am Soc Hortic Sci 34:19–21
Manganaris AG, Alston FH (1987) Inheritance and linkage relationships of glutamate oxaloacetate transaminase isoenzymes in apple. 1. The gene Got-1, a marker for the S-incompatibility locus. Theor Appl Genet 74:154–161
O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
Sassa H, Hirano H, Ikehashi H (1992) Self-incompatibility-related RNases in styles of Japanese pear (Pyrus serotina Rehd.). Plant Cell Physiol 33:811–814
Sassa H, Hirano, H, Ikehashi H (1993) Identification and characterization of stylar glycoproteins associated with self-incompatibility genes of Japanese pear, Pyrus serotina Rehd. Mol Gen Genet 241:17–25
Sims TL (1993) Genetic regulation of self-incompatibility. Crit Rev Plant Sci 12:129–167
Terami H, Torikata H, Shimazu Y (1946) Analysis of the sterility factors existing in varieties of the Japanese pear (Pyrus serotina Rehd.). Studies Hortic Inst Kyoto Imp Univ 3:267–271 (in Japanese)
Yamada M, Suzuki C, Ishiyama M, Sato T, Nakamura Y, Ishizawa K (1971) Fruit set by crossor self-pollination in apple. Tohoku Agric Res 12:282–285 (in Japanese)
Author information
Authors and Affiliations
Additional information
Communicated by H. F. Linskens
Rights and permissions
About this article
Cite this article
Sassa, H., Mase, N., Hirano, H. et al. Identification of self-incompatibility-related glycoproteins in styles of apple (Malus x domestica). Theoret. Appl. Genetics 89, 201–205 (1994). https://doi.org/10.1007/BF00225142
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225142