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Development of a PCR assay and marker-assisted transfer of leaf rust resistance gene Lr58 into adapted winter wheats

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Abstract

Leaf rust resistance gene Lr58 derived from Aegilops triuncialis L. was transferred to the hard red winter wheat (HRWW) cultivars Jagger and Overley by standard backcrossing and marker-assisted selection (MAS). A co-dominant PCR-based sequence tagged site (STS) marker was developed based on the sequence information of the RFLP marker (XksuH16) diagnostically detecting the alien segment in T2BS·2BL-2tL(0.95). STS marker Xncw-Lr58-1 was used to select backcross F1 plants with rust resistance. The co-dominant marker polymorphism detected by primer pair NCW-Lr58-1 efficiently identified the homozygous BC3F2 plants with rust resistance gene Lr58. The STS marker Xncw-Lr58-1 showed consistent diagnostic polymorphism between the resistant source and the wheat cultivars selected by the US Wheat Coordinated Agricultural Project. The utility and compatibility of the STS marker in MAS programs involving robust genotyping platforms was demonstrated in both agarose-based and capillary-based platforms. Screening backcross derivatives carrying Lr58 with various rust races at seedling stage suggested the transferred rust resistance in adapted winter wheats is stable in both cultivar backgrounds. Lr58 in adapted winter wheat backgrounds could be used in combination with other resistance genes in wheat rust resistance breeding.

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Abbreviations

MAS:

Marker assisted selection

STS:

Sequence tagged sites

RFLP:

Restriction fragment length polymorphism

HRWW:

Hard red winter wheat

CAP:

Co-ordinated agricultural project

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Acknowledgments

This research was supported by the North Carolina Agricultural Research Service and Kansas Wheat Commission. We thank Dr. Robert Bowden (USDA-ARS) for providing the rust cultures. We extend a special note of thanks to Duane Wilson (KSU) and Jared Smith (NCSU) for their excellent technical assistance.

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Authors and Affiliations

  1. Crop Science Department, North Carolina State University, Raleigh, NC, 27695, USA

    Vasu Kuraparthy & Shilpa Sood

  2. USDA-ARS, Regional Small Grains Genotyping Laboratory, North Carolina State University, Raleigh, NC, 27695, USA

    Gina-Brown Guedira

  3. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA

    Bikram S. Gill

Authors
  1. Vasu Kuraparthy
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  2. Shilpa Sood
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  3. Gina-Brown Guedira
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  4. Bikram S. Gill
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Correspondence to Vasu Kuraparthy.

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Kuraparthy, V., Sood, S., Guedira, GB. et al. Development of a PCR assay and marker-assisted transfer of leaf rust resistance gene Lr58 into adapted winter wheats. Euphytica 180, 227–234 (2011). https://doi.org/10.1007/s10681-011-0383-4

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  • DOI: https://doi.org/10.1007/s10681-011-0383-4

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