Abstract
The leaf rust resistance gene Lr41 in wheat germplasm KS90WGRC10 and a resistance gene in wheat breeding line WX93D246-R-1 were transferred to Triticum aestivum from Aegilops tauschii and Ae. cylindrica, respectively. The leaf rust resistance gene in WX93D246-R-1 was located on wheat chromosome 2D by monosomic analysis. Molecular marker analysis of F2 plants from non-critical crosses determined that this gene is 11.2 cM distal to marker Xgwm210 on the short arm of 2D. No susceptible plants were detected in a population of 300 F2 plants from a cross between WX93D246-R-1 and TA 4186 (Lr39), suggesting that the gene in WX93D246-R-1 is the same as, or closely linked to, Lr39. In addition, no susceptible plants were detected in a population of 180 F2 plants from the cross between KS90WGRC10 and WX93D246-R-1. The resistance gene in KS90WGRC10, Lr41, was previously reported to be located on wheat chromosome 1D. In this study, no genetic association was found between Lr41 and 51 markers located on chromosome 1D. A population of 110 F3 lines from a cross between KS90WGRC10 and TAM 107 was evaluated with polymorphic SSR markers from chromosome 2D and marker Xgdm35 was found to be 1.9 cM proximal to Lr41. When evaluated with diverse isolates of Puccinia triticina, similar reactions were observed on WX93D246-R-1, KS90WGRC10, and TA 4186. The results of mapping, allelism, and race specificity test indicate that these germplasms likely have the same gene for resistance to leaf rust.
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Acknowledgements
We thank Duane Wilson for excellent technical assistance. G.S. Chahal, S. Brooks, and J.C. Nelson are acknowledged for their helpful reviews and useful comments on the manuscript. We thank Marla Hall for providing Fig. 1. This research was supported by grants from Kansas Wheat Commission, USDA-IFAFS contract number 2001-04462, the Agriculture Biotechnology Program of the Texas Higher Education Coordinating Board, and the Texas Grain Program.
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Contribution number 03-348-J from the Kansas Agricultural Experimental Station, Manhattan, Kansas
Communicated by J. Dvorak
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Singh, S., Franks, C.D., Huang, L. et al. Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located on wheat chromosome 2DS. Theor Appl Genet 108, 586–591 (2004). https://doi.org/10.1007/s00122-003-1477-8
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DOI: https://doi.org/10.1007/s00122-003-1477-8