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Development of co-dominant KASP markers co-segregating with Ug99 effective stem rust resistance gene Sr26 in wheat

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Abstract

Stem rust of wheat, caused by Puccinia graminis f. sp. tritici (Pgt), is a threat to global food security due to its ability to cause total crop failures. The Pgt race TTKSK (Ug99) and its derivatives detected in East Africa carry virulence for many resistance genes present in modern cultivars. However, stem rust resistance gene Sr26 remains effective to all races of Pgt worldwide. Sr26 is carried on the Agropyron elongatum (syn. Thinopyrum ponticum) segment 6Ae#1L translocated to chromosome 6AL of wheat. In this study, a recombinant inbred line (RIL) population derived from a cross between the landrace Aus27969 and Avocet S, which carries Sr26, was used to develop co-dominant kompetitive allele-specific polymerase chain reaction (KASP) markers that co-segregate with Sr26. Four KASP markers (sunKASP_216, sunKASP_218, sunKASP_224 and sunKASP_225) were also shown to co-segregate with Sr26 in four additional RIL populations. When tested on Australian cultivars and breeding lines, these markers amplified alleles alternate to that linked with Sr26 in all cultivars known to lack this gene and Sr26-linked alleles in cultivars and genotypes known to carry Sr26. Genotypes WA-1 and WA-1/3*Yitpi carrying the shortest Sr26 translocation segment were positive only for markers sunKASP_224 and sunKASP_225. Our results suggest the four KASP markers are located on the original translocation and sunKASP_224 and sunKASP_225 are located on the shortened version. Therefore, sunKASP_224 and sunKASP_225 can be used for marker-assisted pyramiding of Sr26 with other stem rust resistance genes to achieve durable resistance in wheat.

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Acknowledgments

All authors thank the Grains Research and Development Corporation (GRDC) Australia for financial support through the Australian Cereal Rust Control Program.

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Author notes

  1. Naeela Qureshi and Pakeerathan Kandiah contributed equally to this work.

Authors and Affiliations

  1. The University of Sydney Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia

    Naeela Qureshi, Pakeerathan Kandiah, Mesfin Kebede Gessese, Vallence Nsabiyera, Vanessa Wells, Prashanth Babu, Harbans Bariana & Urmil Bansal

  2. Department of Agricultural Biology, Faculty of Agriculture, University of Jaffna, Jaffna, Sri Lanka

    Pakeerathan Kandiah

  3. Agriculture Victoria Research, AgriBio, Centre for Agribioscience, Bundoora, VIC, 3083, Australia

    Debbie Wong & Matthew Hayden

  4. School of Applied Systems Biology, La Trobe University, Bundoora, VIC, Australia

    Matthew Hayden

Authors
  1. Naeela Qureshi
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  2. Pakeerathan Kandiah
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  10. Urmil Bansal
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Contributions

UB and HB conceived the research idea; PK developed RIL population; NQ mapped resistance locus using KASP markers; MG, VN, and VW developed RIL populations used in validation; PB phenotyped RIL populations; DW and MH generated targeted GBS map; NQ wrote the manuscript; UB, HB, and MH edited the manuscript.

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Correspondence to Urmil Bansal.

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The authors declare that they have no conflict of interest.

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Key message

This study reports co-dominant markers sunKASP_224 and sunKASP_225 located on the shortest translocated segment from Thinopyrum ponticum (6#AE) for Ug99 effective stem rust resistance gene Sr26.

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Qureshi, N., Kandiah, P., Gessese, M.K. et al. Development of co-dominant KASP markers co-segregating with Ug99 effective stem rust resistance gene Sr26 in wheat. Mol Breeding 38, 97 (2018). https://doi.org/10.1007/s11032-018-0854-6

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