Skip to main content
SpringerLink
Log in

Development and characterization of two new Triticum aestivum–Dasypyrum villosum Robertsonian translocation lines T1DS·1V#3L and T1DL·1V#3S and their effect on grain quality

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Dasypyrum villosum (L.) Candargy is a diploid, wild relative of bread wheat (Triticum aestivum L.). Previous studies showed that D. villosum chromosome 1V has genes that encode seed storage proteins that may be used to enhance the grain quality of bread wheat. As a first step in genetic transfer, the present study was initiated to develop compensating Robertsonian translocations involving wheat chromosome 1D and D. villosum chromosome 1V and to analyze their effects on grain quality. A monosomic 1D stock was crossed with the disomic addition stock DA1V#3 and the double monosomic plants (20″ + 1D′ + 1V#3′) were self pollinated. Two co-dominant STS markers (BE499250 and BE591682) polymorphic for the short arm of 1V#3S and two dominant STS markers (BE518358 and BE585781) polymorphic for the long arm of 1V#3L were developed to screen a large number of progeny to identify plants that had only the 1V#3S or 1V#3L arms. Five compensating Robertsonian heterozygous translocations, two (plants #56 and #83) for the short arm (T1DL·1V#3S) and three (plants #7, #123, and #208) for the long arm (T1DS·1V#3L) were identified from 282 F2 plants and confirmed by genomic in situ hybridization and C-banding analyses. Two homozygous translocations T1DL·1V#3S (plants #14 and #39) were identified from 52 F3 plants derived from F2 plant #83. Four homozygous translocations T1DS·1V#3L (plants #3, #22, #29, and #30) were identified from 68 F3 plants derived from F2 plant #208. The homozygous translocation T1DL·1V#3S had a significantly higher (37.4 ml) and T1DS·1V#3L had significantly lower (10 ml) Zeleny sedimentation values compared to Chinese Spring wheat (30.7 ml). Our results showed that 1V#3S increased gluten strength and enhanced wheat quality, but 1V#3L decreased gluten strength and did not enhance wheat quality.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • AACC (1995) Approved methods of the American association of cereal chemists, 9th edn. AACC, St. Paul

    Google Scholar 

  • Blanco A, Simeone R, Tanzarella OA (1983) Morphology and chromosome pairing of a hybrid between Triticum durum Desf. and Haynaldia villosa (L.) Schur. Theor Appl Genet 64:333–337

    Article  Google Scholar 

  • Blanco A, Resta P, Simeone R, Parmar S, Shewry PR, Sabelli P, Lafiandra D (1991) Chromosomal location of seed storage protein genes in the genome of Dasypyrum villosum (L.) Candargy. Theor Appl Genet 82:358–362

    Article  CAS  Google Scholar 

  • Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ (1995) Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91:1125–1128

    Article  Google Scholar 

  • Chen X, Shi AN, Shang LM, Leath S, Murphy JP (1997) The resistance of H. villosa to powdery mildew isolates and its expression in wheat background. Acta Phytopathol Sin 27:17–22

    Google Scholar 

  • Chen J, Deng GB, Yu MQ, Ren ZL (2001) Study on the resistance genetic and cytogenetic behaviour of 6VS in a wheat-Dasypyrum villosum telosomic addition line. J Sichuan Agric Univ 19:1–5

    Google Scholar 

  • Chen Q, Conner RL, Li H, Laroche A, Graf RJ, Kuzyk AD (2002) Expression of resistance to stripe rust, powdery mildew and the wheat curl mite in Triticum aestivum · Haynaldia villosa lines. Can J Plant Sci 82:451–456

    Google Scholar 

  • Davies PA, Pallotta MA, Driscoll CJ (1985) Centric fusion between nonhomologous rye chromosomes in wheat. Can J Genet Cytol 27:627–632

    Google Scholar 

  • De Pace C, Snidaro D, Ciaffi M, Vittori D, Ciofo A, Cenci A, Tanzarella OA, Qualset CO, Scarascia Mugnozza GT (2001) Introgression of Dasypyrum villosum chromatin into common wheat improves grain protein quality. Euphytica 117:67–75

    Article  Google Scholar 

  • Friebe B, Cermeno MC, Zeller FJ (1987) C-banding polymorphism and the analysis of nucleolar activity in Dasypyrum villosum (L.) Candargy, its added chromosomes to hexaploid wheat and the amphiploid Triticum dicoccum–D. villosum. Theor Appl Genet 73:337–342

    Article  Google Scholar 

  • Friebe B, Zhang P, Linc G, Gill BS (2005) Robertsonian translocations in wheat arise by centric misdivision of univalents at anaphase I and rejoining of broken centromeres during interkinesis of meiosis II. Cytogenet Genome Res 109:293–297

    Article  CAS  PubMed  Google Scholar 

  • Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome 34:830–839

    Google Scholar 

  • Hyde BB (1953) Addition of individual Haynaldia villosa chromosomes to hexaploid wheat. Am J Bot 40:174–182

    Article  Google Scholar 

  • Li H, Chen X, Xin ZY, Ma YZ, Xu HJ, Chen XY, Jia X (2005) Development and identification of wheat-Haynaldia villosa T6DL·6VS chromosome translocation lines conferring resistance to powdery mildew. Plant Breed 124:203–205

    Article  CAS  Google Scholar 

  • Li G, Chen PD, Zhang S, Wang X, He Z, Zhang Y, Zhao H, Huang H, Zhou X (2007) Effects of the 6VS·6AL translocation on agronomic traits and dough properties of wheat. Euphytica 155:305–313

    Article  Google Scholar 

  • Linde-Laursen I, Jensen HP, Jørgensen JH (1973) Resistance of Triticeae, Aegilops and Haynaldia species to the take–all fungus, Gaeumannomyces graminis. Z Pflanzenzücht 70:20–213

    Google Scholar 

  • Liu DJ, Chen PD, Pei GZ, Wang YN, Qiu BX, Wang SL (1988) Transfer of Haynaldia villosa chromosomes into Triticum aestivum. In: Miller TE, Koebner RMD (eds) Proceedings of the 7th international wheat genetics symposium, vol 1. IPSR, Cambridge, pp 355–361

  • Lukaszewski AJ (1993) Reconstruction in wheat of complete chromosomes 1B and 1R from the 1RS.1BL translocation of ‘Kavkas’ origin. Genome 36:821–824

    Article  CAS  PubMed  Google Scholar 

  • Lukaszewski AJ (1994) Manipulation of the genome by chromosome breakage. In: Gill BS, Raupp WJ (eds) Proceedings of the US-Japan symposium, classical and molecular cytogenetic analysis, March 1994, Manhattan, KS, USA, pp 136–139

  • Lukaszewski AJ (1997) Further manipulation by centric misdivision of the 1RS·1BL translocation in wheat. Euphytica 94:257–261

    Article  Google Scholar 

  • Montebove L, De Pace C, Jan CC, Scarascia-Mugnozza GT, Qualset CO (1987) Chromosomal location of isozyme and seed storage protein genes in Dasypyrum villosum (L.) Candargy. Theor Appl Genet 73:836–845

    Article  CAS  Google Scholar 

  • Murray TD, De la Pena RC, Yildirim A, Jones SS (1994) A new source of resistance to Pseudocercosporella herpotrichoides cause of eyespot disease of wheat located on chromosome 4V of Dasypyrum villosum. Plant Breed 113:281–286

    Article  Google Scholar 

  • Qi LL, Chen PD, Liu DJ, Zhou B, Zhang SZ et al. (1993) Development of translocation lines of Triticum aestivum with powdery mildew resistance introduced from Haynaldia villosa. In: Li ZS, Xin ZY (eds) Proceedings of the 8th international wheat genetics symposium. Chinese Agricultural Scientech Press, Beijing, pp 333–337

  • Qi LL, Echalier B, Friebe B, Gill BS (2003) Molecular characterization of a set of wheat deletion stocks for using in chromosome bin mapping of ESTs. Funct Integr Genomics 3:39–55

    CAS  PubMed  Google Scholar 

  • Qi LL, Friebe B, Zhang P, Gill BS (2007) Homoeologous recombination, chromosome engineering and crop improvement. Chromosome Res 15:3–19

    Article  CAS  PubMed  Google Scholar 

  • Qi LL, Pumphrey MO, Friebe B, Chen PD, Gill BS (2008) Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theor Appl Genet 117:1155–1166

    Article  CAS  PubMed  Google Scholar 

  • Robertson WMRB (1916) Chromosome studies. I. Taxonomic relationships shown in the chromosomes of Tettegidae and Acrididiae: V-shaped chromosomes and their significance in Acrididae, Locustidae and Grillidae: chromosomes and variations. J Morphol 27:179–331

    Article  Google Scholar 

  • Sears ER (1952) Misdivision of univalents in common wheat. Chromosoma 4:535–550

    Article  CAS  PubMed  Google Scholar 

  • Sears ER (1953) Addition of the genome of H. villosa to T. aestivum. Am J Bot 40:168–174

    Article  Google Scholar 

  • Shewry PR, Parmar S, Pappin DJC (1987) Characterization and genetic control of the prolamins of Haynaldia villosa: relationship to cultivated species of the Triticeae (rye, wheat and barley). Biochem Genet 25:309–325

    Article  CAS  PubMed  Google Scholar 

  • Zhang Q, Li Q, Wang X, Wang H, Lang S, Wang Y, Wang S, Chen P, Liu D (2005) Development and characterization of a Triticum aestivum–Haynaldia villosa translocation line T4VS-4DL conferring resistance to wheat spindle streak mosaic virus. Euphytica 145:317–320

    Article  CAS  Google Scholar 

  • Zhong GY, Qualset CO (1993) Allelic diversity of high-molecular-weight glutenin protein subunits in natural populations of Dasypyrum villosum (L.) Candargy. Theor Appl Genet 86:851–858

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank John Raupp for editorial and Duane Wilson for excellent technical assistance. This research was supported by grants from the Kansas Wheat Commission, the Kansas Crop Improvement Association, and a special USDA-CSREES grant to the Wheat Genetic and Genomic Resources Center. This research was also supported by a grant from the China Scholarship Council and Tang Zhong Ying Wheat Breeding Foundation of Northwest Agriculture and Forestry University. This paper is contribution number 10-255-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, Kansas.

Author information

Authors and Affiliations

  1. College of Agronomy, Northwest Agriculture & Forestry University, Yangling, Shaanxi, 712100, China

    Wanchun Zhao, Xiang Gao, Gaisheng Zhang, Jian Dong & Qijiao Chen

  2. USDA-ARS, Northern Crop Science Laboratory, Fargo, ND, 58105-5677, USA

    Lili Qi

  3. Department of Plant Pathology, Wheat Genetic and Genomic Resources Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506, USA

    Bernd Friebe & Bikram S. Gill

Authors
  1. Wanchun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lili Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gaisheng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jian Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qijiao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Bernd Friebe
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Bikram S. Gill
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bernd Friebe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, W., Qi, L., Gao, X. et al. Development and characterization of two new Triticum aestivum–Dasypyrum villosum Robertsonian translocation lines T1DS·1V#3L and T1DL·1V#3S and their effect on grain quality. Euphytica 175, 343–350 (2010). https://doi.org/10.1007/s10681-010-0177-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-010-0177-0

Keywords

Search

Navigation