Abstract
Dasypyrum villosum (L.) Candargy (2n=14, V genome) is a wild, allogamous, diploid grass species that is a potential genetic resource for wheat improvement. The diversity of high-molecular-weight (HMW) glutenin subunits of the seed storage proteins of this species was examined in populations sampled in their natural habitats in Italy and Yugoslavia where the species is widely distributed. The results of selfed progeny tests confirmed that the allelic variation of HMW-glutenin subunits in D. villosum is controlled by a single locus (Glu-V1). Fourteen alleles at Glu-V1 were found among 982 individuals representing 12 populations from Italy and two from Yugoslavia, with a mean of seven alleles per population. Among the 14 Glu-V1 alleles, one produced no HMW-glutenin subunits, ten coded for a single subunit, and three for two subunits. The mobilities of all the subunits in SDS-PAGE gels were greater than that of reference subunit 7 of Triticum aestivum cv Chinese Spring. Eight of the alleles were relatively abundant (mean frequency over all populations ranged from 0.08 to 0.17) and distributed widely among the 14 populations (8 to 14); five of the alleles were rare (0.003 to 0.021) and found in only 1 to 5 populations. The frequencies of two alleles could not be individually estimated because of the similar electrophoretic mobility of their subunits. The multiple-allelic diversity at Glu-V1 was high (He ranged from 0.700 to 0.857) but similar from population to population. Overall, about 7% of the total allelic variation was distributed among populations (Gst=0.072), and more than 90% within populations. Whether the allelic variation at Glu-V1 is subject to natural selection is unknown, but the discovery of the homozygous null Glu-V1 alleles in the present study may be useful in pursuing this question. The multiple-allelic diversity in Glu-V1 presents the plant breeder with an opportunity to evaluate and select the most useful alleles for transfer to wheat. The importance of an evaluation genetic diversity in a wild species before interspecific gene transfers are attempted is well illustrated in this study.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blanco A, Simeone R, Resta P (1987) The addition of Dasypyrum Villosum (L.) Candargy chromosomes to durum wheat (Triticum durum Desf.). Theor Appl Genet 74:328–333
Blanco A, Resta P, Simeone R, Parmar S, Shewry PR, Sabelli P, Lafiandra D (1991) Chromosomal location of seed storage protein genes in the genome of Dasypyrum villosum (L.) Candargy. Theor Appl Genet 82:358–362
Brown AHD (1979) Enzyme polymorphism in plant populations. Theor Pop Biol 15:1–42
Brown AHD, Allard RW (1970) Estimation of the mating system in open-pollinated maize populations using isozyme polymorphisms. Genetics 66:133–145
De Pace C (1987) Genetic variability in natural populations of Dasypyrum villosum (L.) Candargy. PhD Dissertation, University of California, Davis
De Pace C, Montebove L, Delre V, Jan CC, Qualset CO, Scarascia Mugnozza GT (1988) Biochemical versatility of amphiploids derived from crossing Dasypyrum villosum Candargy and wheat: genetic control and phenotypic aspects. Theor Appl Genet 76:513–529
De Pace C, Paolini R, Scarascia Mugnozza GT, Qualset CO, Delre V (1990) Evaluation and utilization of Dasypyrum villosum as a genetic resource for wheat improvement. In: Srivastava JP, Damania AB (eds) Wheat genetic resources: meeting diverse needs. J Wiley and Sons, New York, pp 279–290
Doebley JF, Goodman MM, Stuber CW (1985) Isozyme variation in the races of maize from Mexico. Am J Bot 72:629–639
Fernàndez-Calvin B, Orellana J (1990) High-molecular-weight glutenin subunit variation in the Sitopsis section of Aegilops. Implications for the origin of the B genome of wheat. Heredity 65:455–463
Fullington JG, Cole EW, Kasarda DD (1983) Quantitative SDS-PAGE of total proteins from different wheat varieties: effects of protein content. Cereal Chem 50:65–70
Gepts P (1990) Genetic diversity of seed storage proteins in plants. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding, and genetic resources. Sinauer Associates Inc Publishers, Sunderland, Massachusetts, pp 64–82
Jan CC, De Pace C, McGuire PE, Qualset CO (1986) Hybrids and amphiploids of Triticum aestivum L. and T. turgidum L., with Dasypyrum villosum (L.) Candargy. Z Pflanzenzüchtg 96:97–106
Lagudah ES, Halloran GM (1988) Phylogenetic relationships of Triticum tauschii, the D genome donor to hexaploid wheat. I. Variation in HMW subunits of glutenin and gliadin. Theor Appl Genet 75:592–598
Lagudah ES, MacRitchie F, Halloran GM (1987) The influence of high-molecular-weight subunits of glutenin from Triticum tauschii on flour quality of synthetic hexaploid wheat. J Cereal Sci 5:129–138
Law CN, Payne PI (1983) Review: genetical aspects of breeding for improved grain protein content and type in wheat. J Cereal Sci 1:79–93
Lawrence GJ, Shepherd KW (1980) Variation in glutenin protein subunits of wheat. Aust J Biol Sci 33:221–223
Levy AA, Feldman M (1988) Ecogeographical distribution of HMW-glutenin alleles in populations of the wild tetraploid wheat Triticum turgidum var. dicoccoides. Theor Appl Genet 75:651–658
Levy AA, Galili G, Feldman M (1988) Polymorphism and genetic control of high-molecular-weight glutenin subunits in wild tetraploid wheat Triticum turgidum var. dicoccoides. Heredity 61:63–72
Liu D, Chen P, Pei G, Wang Y (1983) Studies on transfer of genetic materials from Haynaldia villosa to Triticum aestivum. Acta Genet Sin 10:103–113
Mansur LM, Qualset CO, Kasarda DD, Morris R (1990) Effects of ‘Cheyenne’ chromosomes on milling and baking quality in ‘Chinese Spring’ wheat in relation to glutenin and gliadin storage proteins. Crop Sci 30:593–602
Montebove L, De Pace C, Jan CC, Scarascia Mugnozza GT, Qualset CO (1987) Chromosomal location of isozyme and seed storage protein genes in Dasypyrum villosum (L.) Candargy. Theor Appl Genet 73:836–845
Nei M (1975) Molecular population genetics and evolution. North Holland, Amsterdam
Nevo E, Payne PI (1987) Wheat storage proteins: diversity of HMW-glutenin subunits in wild emmer from Israel. Theor Appl Genet 74:827–836
Nevo E, Golenberg E, Beiles A, Brown AHD, Zohary D (1982) Genetic diversity and environmental associations of wild wheat, Triticum dicoccoides in Israel. Theor Appl Genet 62:241–254
Nevo E, Beiles A, Storch N, Doll H, Andersen B (1983) Microgeographic edaphic differentiation in hordein polymorphisms of wild barley. Theor Appl Genet 64:123–132
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Lawrence GL (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 1:29–33
Payne PI, Corfield KG, Holt LM, Blackman JA (1981) Correlations between the inheritance of certain high-molecularweight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J Sci Food Agric 32:51–60
Qualset CO, McGuire PE, Stupar M, Pavicevic L (1984) Collection of Dasypyrum villosum (syn Haynaldia villosa), Aegilops spp, and Hordeum bulbosum in Yugoslavia. University of California, Davis Agronomy Progress Report, No. 157
Rogers WJ, Sayers EJ, Harris PA, Law CN, Payne PI (1987) Effect of allelic variation for glutenin subunits and gliadins on bread-making quality, exploitation of novel alleles found in wild relatives of wheat. In: Lasztity R, Bekes F (eds) Proc 3rd Int Gluten Workshop, Budapest, Hungary. World Scientific Publishers, Singapore, pp 45–56
Rousset M, Carrillo JM, Qualset CO, Kasarda DD (1992) Use of recombinant inbred lines of wheat for study of associations of high-molecular-weight glutenin subunit alleles to quantitative traits. 2. Milling and bread-making quality. Theor Appl Genet 83:403–412
Scott, PR (1981) Variation in host susceptibility. In: Asher MJC, Shipton PJ (eds) Ecology and control of take-all. Academic Press, London
Sears ER (1953) Addition of the genome of Haynaldia villosa to Triticum aestivum. Am J Bot 40:168–174
Shewry PR, Parmar S, Pappin DJC (1987) Characterization and genetic control of the prolamins of Haynaldia villosa: relationship to cultivated species of the Triticeae (rye, wheat and barley). Biochem Genet 25:309–325
Shrewy PR, Sabelli PA, Parmar S, Lafianda D (1991) α-type prolamins are encloded by genes on chromosomes 4Ha and 6Ha of Haynaldia villosa Schur (syn. Dasypyrum villosum L). Biochem Genet 29:207–211
Sun M, Corke H (1992) Population genetics of colonizing success of weedy rye in northern California. Theor Appl Genet 83:321–329
Author information
Authors and Affiliations
Additional information
Communicated by G. E. Hart
Rights and permissions
About this article
Cite this article
Zhong, G.Y., Qualset, C.O. Allelic diversity of high-molecular-weight glutenin protein subunits in natural populations of Dasypyrum villosum (L.) Candargy. Theoret. Appl. Genetics 86, 851–858 (1993). https://doi.org/10.1007/BF00212612
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00212612