Summary
The zymogram phenotypes of glucose-phosphate isomerase (GPI), alcohol dehydrogenase-1 (ADH-1), glutamate oxaloacetate transaminase (GOT), superoxide dismutase (SOD), lipoxygenase (LPX), esterase (EST) and the banding patterns of gliadin and glutenin seed storage proteins were determined for Triticum aestivum cv. ‘Chinese Spring’ (CS), Dasypyrum villosum, the octoploid amphiploid T. aestivum cv. ‘Chinese Spring’ D. villosum (CS × v) (2n=8x=56; AABBDDVV), and for five CS-D. villosum disomic addition lines. The genes Gpi-V1, Adh-V1, Got-V2, and Sod-V2 coding for GPI-1, ADH-1, GOT-2, and SOD-2 isozymes were located in D. villosum on chromosome 1V, 4V, 6V, and 7V, respectively. Genes coding for gliadin- and glutenin-like subunits are located in D. villosum chromosomes 1V. There are no direct evidence for chromosomal location of genes coding for GOT-3, EST-1 and LPX-2 isozymes. The linkage between genes coding for glutenin-like proteins and GPI-1 isozymes in chromosome 1V is evidence of homoeology between chromosome 1V and the chromosomes of homoeologous group 1 in wheat.
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References
Barber HN, Driscoll CJ, Long PM, Vickery RS (1968) Protein genetics of wheat and homoeologous relationships of chromosomes. Nature 218:450–452
Barber HN, Driscoll CJ, Long PM, Vickery RS (1969) Gene similarity of the Triticinae and the study of segmental interchanges. Nature 222:897–898
Arus P, Orton TJ (1984) Inheritance patterns and linkage relationships of eight genes of celery. J Hered 75:11–14
Berg von KH (1934) Weitere Studien am fertiliten konstanten Artbastard T. turgidovillosum und seinen Verwandten. 2. Cytologische Untersuchungen an Triticum turgidovillosum und seinen Eltern. Z Indukt Abstamm-Vererbungsl 67:342–373
Chojecki AJS, Gale MD, Holt LM, Payne PI (1983) The intrachromosomal mapping of a glucose phospate isomerase structural gene, using allelic variation among stocks of ‘Chinese Spring’ wheat. Genet Res 41:221–226
De Pace C, Montebove L, Delre V (1986) Genetic control of GPI, GOT, ADH, SOD, LPX isozymes and seed storage proteins in amphiploids derived from crossing Dasypyrum villosum and wheat (in prep)
Fullington JG, Cole EW, Kasarda DD (1983) Quantitative sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total proteins extracted from different wheat varieties: effect of protein content. Cereal Chem 60:65–71
Hart GE (1970) Evidence for triplicate genes for alcohol dehydrogenase in hexaploid wheat. Proc Natl Acad Sci USA 66:1136–1141
Hart GE (1975) Glutamate oxaloacetate transaminase isozymes of Triticum: evidence for multiple systems of triplicate structural genes in hexaploid wheat. In: Markert Cl (ed) Isozymes, vol 3. Developmental biology. Academic Press, New York, pp 637–657
Hart GE (1979 a) Evidence for a triplicate set of glucose-phosphate isomerase structural genes in hexaploid wheat. Biochem Genet 17:585–598
Hart GE (1979 b) Genetical and chromosomal relationships among the wheats and their relatives. Stadler Genet Symp 11:9–29
Hart GE (1982) Manual describing techniques, buffers, and staining mixtures used for electrophoretic study of wheat and its relatives. Texas A & M University, USA
Hart GE, Langston PJ (1977) Chromosomal location and evolution of isozyme structural genes in hexaploid wheat. Heredity 39:263–277
Hart GE, Tuleen NA (1983) Introduction and characterization of alien genetic material. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier Sci Publ, Amsterdam, pp 339–362
Jaaska V (1980) Electrophoretic survey of seedling esterases in wheat in relation to their phylogeny. Theor Appl Genet 6:273–284
Jaaska V (1982) Isoenzymes of superoxide dismutase in wheats and their relatives: alloenzyme variation. Biochem Physiol Pflanz 177:747–755
Jan CC, De Pace C, McGuire PE, Qualset CO (1986) Hybrids and amphiploids of Triticum aestivum L. and T. turgidum L. with Dasypyrum villosum (L.) Candargy. Z Pflanzenzücht 96:97–106
Lafiandra D, Kasarda DD, Morris R (1984) Chromosomal assignement of genes for wheat gliadin protein components of the cultivars ‘Cheyenne’ and ‘Chinese Spring’ by two dimensional (two-pH) electrophoresis. Theor Appl Genet 68:531–539
Law CN, Payne PI (1983) Genetical aspects of breeding for improved grain protein content and type in wheat. J Cereal Sci 1:79–83
Mecham DK, Kasarda DD, Qualset CO (1978) Genetic aspects of wheat gliadin proteins. Biochem Genet 16:831–853
Montebove L (1985) Study of the genetical and morphological characters of Triticum × Dasypyrum amphiploids (in Italian). B Sc Thesis Universitá degli studi della Tuscia, Viterbo, Italy
Neuman PR, Hart GE (1986) Genetic control of the mitochondrial form of superoxide dismutase in hexaploid wheat. Biochem Genet (in press)
Payne PI, Holt LM, Jackson EA, Law CN (1984) Wheat storage protein: their genetics and their potential for manipulation by plant breeding. Philos Trans R Soc London, B 304:359–371
Qualset CO, De Pace C, Jan CC, Scarascia Mugnozza GT, Tanzarella OA, Greco B (1981) Haynaldia villosa (L.) Schur: a species with potential use in wheat breeding. Am Soc Agron Abstr p 70
Shumaker KM, Allard RW, Kahler AL (1984) Cryptic variability at enzyme loci in three plant species, Avena barbata, Hordeum vulgare, and Zea mais. J Hered 73:86–90
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Communicated by F.Mechelke and H. F.Linskens
Research supported by the National Research Council (Italy) and National Science Foundation (USA). International cooperative project, Grant No. 85.01504.06 (CNR)
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Montebove, L., De Pace, C., Jan, C.C. et al. Chromosomal location of isozyme and seed storage protein genes in Dasypyrum villosum (L.) Candargy. Theoret. Appl. Genetics 73, 836–845 (1987). https://doi.org/10.1007/BF00289388
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DOI: https://doi.org/10.1007/BF00289388