Skip to main content
SpringerLink
Log in

Identification of genomic regions associated with seed dormancy in white-grained wheat

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Pre-harvest sprouting (PHS) in developing wheat (Triticum aestivum L.) spikes is stimulated by cool and wet weather and leads to a decline in grain quality. A low level of harvest-time seed dormancy is a major factor for PHS, which generally is a larger problem in white-grained as compared to red-grained wheat. We have in this study analyzed seed dormancy levels at the 92nd Zadok growth stage of spike development in a doubled-haploid (DH) white wheat population and associated variation for the trait with regions on the wheat genome. The phenotypic data was generated by growing the parent lines Argent (non-dormant) and W98616 (dormant) and 151 lines of the DH population in the field during 2002 and 2003, at two locations each year, followed by assessment of harvest-time seed dormancy by germination tests. A genetic map of 2681 cM was constructed for the population upon genotyping 90 DH lines using 361 SSR, 292 AFLP, 252 DArT and 10 EST markers. Single marker analysis of the 90 genotyped lines associated regions on chromosomes 1A, 2B, 3A, 4A, 5B, 6B, and 7A with seed dormancy in at least two out of the four trials. All seven putative quantitative trait loci (QTLs) were contributed by alleles of the dormant parent, W98616. The strongest QTLs positioned on chromosomes 1A, 3A, 4A and 7A were confirmed by interval mapping and markers at these loci have potential use in marker-assisted selection of PHS resistant white-grained wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

AFLP:

Amplified Fragment Length Polymorphism

DArT:

Diversity Array Technology

DH:

Doubled-haploid

EST:

Expressed sequence tag

PHS:

Pre-harvest sprouting

QTL:

Quantitative trait locus

SSR:

Simple sequence repeats

References

  • Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang S, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughn P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420

    Article  CAS  PubMed  Google Scholar 

  • Anderson JA, Sorrells ME, Tanksley SD (1993) RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. Crop Sci 33:453–459

    CAS  Google Scholar 

  • Båga M, Chodaparambil SV, Limin AE, Pecar M, Fowler DB, Chibbar RN (2007) Identification of quantitative trait loci and associated candidate genes for low-temperature tolerance in cold-hardy winter wheat. Funct Integr Genomics 7:53–68

    Article  PubMed  CAS  Google Scholar 

  • Bailey PC, McKibbin RS, Lenton JR, Holdsworth MJ, Flintham JE, Gale MD (1999) Genetic map locations for orthologous Vp1 genes in wheat and rice. Theor Appl Genet 98:281–284

    Article  CAS  Google Scholar 

  • Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066

    Article  CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Eujayl I, Sorrells ME, Baum M, Wolters P, Powell W (2002) Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat. Theor Appl Genet 104:399–407

    Article  CAS  PubMed  Google Scholar 

  • Faris JD, Laddomada B, Gill BS (1998) Molecular mapping of segregation distortion loci in Aegilops tauschii. Genetics 149:319–327

    CAS  PubMed  Google Scholar 

  • Flintham J, Adlam R, Bassoi M, Holdsworth M, Gale M (2002) Mapping genes for resistance to sprouting damage in wheat. Euphytica 126:39–45

    Article  CAS  Google Scholar 

  • Groos C, Gay G, Perretant MR, Gervais L, Bernard M, Dedryver F, Charmet G (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theor Appl Genet 104:39–47

    Article  CAS  PubMed  Google Scholar 

  • Himi E, Noda K (2005) Red grain colour gene (R) of wheat is a Myb-type transcription factor. Euphytica 143:239–242

    Article  CAS  Google Scholar 

  • Himi E, Mares DJ, Yanagisawa A, Noda K (2002) Effect of grain colour gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. J Exp Bot 53:1569–1574

    Article  CAS  PubMed  Google Scholar 

  • Holdsworth MJ, Bentsink L, Soppe WJJ (2008) Molecular networks regulating Arabidopsis seed maturation, after-ripening, dormancy and germination. New Phytol 179:33–54

    Article  CAS  PubMed  Google Scholar 

  • Howell KA, Narsai R, Carroll A, Ivanova A, Lohse M, Usadel B, Millar AH, Whelan J (2009) Mapping metabolic and transcript temporal switches during germination in rice highlights specific transcription factors and the role of RNA instability in the germination process. Plant Physiol 149:961–980

    Article  CAS  PubMed  Google Scholar 

  • Hucl P, Matus-Cádiz M, Hanson K, Perron C, Tyler R (2005) Development of hard white bread wheat with sprouting resistance. ADF project report (19990102). http://www.agriculture.gov.sk.ca/Projects. pp 67

  • Kammholz SJ, Campbell AW, Sutherland MW, Hollamby GJ, Martin PJ, Eastwood RF, Barclay I, Wilson RE, Brennan PS, Sheppard JA (2001) Establishment and characterisation of wheat genetic mapping populations. Aust J Agric Res 52:1079–1088

    Article  CAS  Google Scholar 

  • Kato K, Nakamura W, Tabiki T, Miura H, Sawada S (2001) Detection of loci controlling seed dormancy on group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 102:980–985

    Article  CAS  Google Scholar 

  • Knox RE, Clarke FR, Clarke JM, Fox SL (2005) Genetic analysis of pre-harvest sprouting in a durum wheat cross. Euphytica 143:261–264

    Article  CAS  Google Scholar 

  • Kulwal PL, Kumar N, Gaur A, Khurana P, Khurana JP, Tyagi AK, Balyan HS, Gupta PK (2005) Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat. Theor Appl Genet 111:1052–1059

    Article  CAS  PubMed  Google Scholar 

  • Lagudah ES, Appels R, Brown AHD, McNeil D (1991) The molecular-genetic analysis of Triticum tauschii, the D-genome donor to hexaploid wheat. Genome 34:375–386

    CAS  Google Scholar 

  • Laurie DA, Bennett MD (1988) The production of haploid wheat plants from wheat × maize crosses. Theor Appl Genet 76:393–397

    Article  Google Scholar 

  • Li C, Ni P, Francki M, Hunter A, Zhang Y, Schibeci D, Li H, Tarr A, Wang J, Cakir M, Yu J, Bellgard M, Lance R, Appels R (2004) Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison. Funct Integr Genomics 4:84–93

    Article  CAS  PubMed  Google Scholar 

  • Lyttle TW (1991) Segregation distorters. Annu Rev Genet 25:511–557

    Article  CAS  PubMed  Google Scholar 

  • Mares D, Mrva K, Cheong J, Williams K, Watson B, Storlie E, Sutherland M, Zou Y (2005) A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theor Appl Genet 111:1357–1364

    Article  CAS  PubMed  Google Scholar 

  • McKibbin RS, Wilkinson MD, Bailey PC, Flintham JE, Andrew LM, Lazzeri PA, Gale MD, Lenton JR, Holdsworth MJ (2002) Transcripts of Vp-1 homologues are misspliced in modern wheat and ancestral species. Proc Natl Acad Sci USA 99:10203–10208

    Article  CAS  PubMed  Google Scholar 

  • Mori M, Uchino N, Chono M, Kato K, Miura H (2005) Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theor Appl Genet 110:1315–1323

    Article  CAS  PubMed  Google Scholar 

  • Nakamura S, Komatsuda T, Miura H (2007) Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy. Theor Appl Genet 114:1129–1139

    Article  CAS  PubMed  Google Scholar 

  • Nicot N, Chiquet V, Gandon B, Amilhat L, Legeai F, Leroy P, Bernard M, Sourdille P (2004) Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs). Theor Appl Genet 109:800–805

    Article  CAS  PubMed  Google Scholar 

  • Noda K, Matsuura T, Maekawa M, Taketa S (2002) Chromosomes responsible for sensitivity of embryo to abscisic acid and dormancy in wheat. Euphytica 123:203–209

    Article  CAS  Google Scholar 

  • Ogbonnaya FC, Imtiaz M, DePauw RM (2007) Haplotype diversity of preharvest sprouting QTLs in wheat. Genome 50:107–118

    Article  CAS  PubMed  Google Scholar 

  • Ogbonnaya FC, Imtiaz M, Ye G, Hearnden PR, Hernandez E, Eastwood RF, van Ginkel M, Shorter SC, Winchester JM (2008) Genetic and QTL analyses of seed dormancy and preharvest sprouting resistance in wheat germplasm CN10955. Theor Appl Genet 116:891–902

    Article  CAS  PubMed  Google Scholar 

  • Ranford JC, Bryce JH, Morris PC (2002) PM19, a barley (Hordeum vulgare L.) gene encoding a putative plasma membrane protein, is expressed during embryo development and dormancy. J Exp Bot 53:147–148

    Article  CAS  PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Singh K, Ghai M, Garg M, Chhuneja P, Kaur P, Schnurbusch T, Keller B, Dhaliwal HS (2007) An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum × T. monococcum RIL population. Theor Appl Genet 115:301–312

    Article  CAS  PubMed  Google Scholar 

  • Singh R, Matus-Cádiz MA, Båga M, Hucl P, Chibbar RN (2008) Comparison of different methods for phenotyping pre-harvest sprouting in white-grained wheat. Cereal Chem 85:238–242

    Article  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Sourdille P, Tavaud M, Charmet G, Bernard M (2001) Transferability of wheat microsatellites to diploid Triticeae species carrying the A, B and D genomes. Theor Appl Genet 103:346–352

    Article  CAS  Google Scholar 

  • Sreenivasulu N, Usadel B, Winter A, Radchuk V, Scholz U, Stein N, Weschke W, Strickert M, Close TJ, Stitt M, Graner A, Wobus U (2008) Barley grain maturation and germination: metabolic pathway and regulatory network commonalities and differences highlighted by new MapMan/PageMan profiling tools. Plant Physiol 146:1738–1758

    Article  CAS  PubMed  Google Scholar 

  • Tondelli A, Francia E, Barabaschi D, Aprile A, Skinner JS, Stockinger EJ, Stanca AM, Pecchioni N (2005) Mapping regulatory genes as candidates for cold and drought stress tolerance in barley. Theor Appl Genet 112:445–454

    Article  PubMed  CAS  Google Scholar 

  • Torada A, Ikeguchi S, Koike M (2005) Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat. Euphytica 143:251–255

    Article  CAS  Google Scholar 

  • van Ooijen JW (2004) MapQTL®5, software for the mapping of quantitative trait loci in experimental populations. Kyazma BV, Wageningen, The Netherlands

    Google Scholar 

  • van Ooijen JW, Voorrips RE (2001) Joinmap®3.0, software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The Netherlands

    Google Scholar 

  • Vardi A (1973) Introgression between different ploidy levels in the wheat group. In: Sears ER, Sears LMS (eds) Proceedings of 4th international wheat genetics symposium. University Missouri Press, Columbia, USA, pp 131–141

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  CAS  PubMed  Google Scholar 

  • Walker-Simmons M (1987) ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84:61–66

    Article  CAS  PubMed  Google Scholar 

  • Wilson ID, Barker GLA, Lu C, Coghill JA, Beswick RW, Lenton JR, Edwards KJ (2005) Alteration of the embryo transcriptome of hexaploid winter wheat (Triticum aestivum cv. Mercia) during maturation and germination. Funct Integr Genomics 5:144–154

    Article  PubMed  Google Scholar 

  • Yu K, Park SJ, Poysa V (2000) Marker assisted selection of common beans for resistance to common bacterial blight: efficacy and economics. Plant Breed 119:411–415

    Article  CAS  Google Scholar 

  • Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421

    Article  Google Scholar 

  • Zanetti S, Winzeler M, Keller M, Keller B, Messmer M (2000) Genetic analysis of pre-harvest sprouting resistance in a wheat × spelt cross. Crop Sci 40:1406–1417

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Canada Research Chairs Program, Canada Foundation for Innovation, Saskatchewan Innovation Fund and Saskatchewan Agriculture and Food are gratefully acknowledged for financial support for this research. R. Singh is also grateful to the Indian Council of Agricultural Research (ICAR), New Delhi, India, for granting study leave for Ph.D.

Author information

Author notes

  1. Rajender Singh

    Present address: Directorate of Wheat Research, Karnal, 132001, India

Authors and Affiliations

  1. Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Rajender Singh, Maria Matus-Cádiz, Monica Båga, Pierre Hucl & Ravindra N. Chibbar

Authors
  1. Rajender Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria Matus-Cádiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monica Båga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pierre Hucl
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ravindra N. Chibbar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ravindra N. Chibbar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, R., Matus-Cádiz, M., Båga, M. et al. Identification of genomic regions associated with seed dormancy in white-grained wheat. Euphytica 174, 391–408 (2010). https://doi.org/10.1007/s10681-010-0137-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-010-0137-8

Keywords

Search

Navigation