Abstract
Seed dormancy is one of the important factors controlling pre-harvest sprouting (PHS) resistance in wheat. We identified a major quantitative trait locus (QTL) for seed dormancy on the long arm of wheat chromosome 4A (4AL) via simple sequence repeat (SSR)-based genetic mapping using doubled haploid lines from a cross between Japanese PHS resistant variety ‘Kitamoe’ and the Alpine non-resistant variety “Münstertaler” (K/M). The QTL explained 43.3% of total phenotypic variation for seed dormancy under greenhouse conditions. SSR markers flanking the QTL were assigned to the chromosome long arm fraction length 0.59–0.66 on the basis of chromosome deletion analysis, suggesting that the gene(s) controlling seed dormancy are probably located within this region. Under greenhouse conditions, the QTL explained 28.5 and 39.0% of total phenotypic variation for seed dormancy in Haruyutaka/Leader (HT/L) and OS21-5/Haruyokoi (O/HK) populations, respectively. However, in field conditions, the effect was relatively low or not significant in both the K/M and HT/L populations. These markers were considered to be widely useful in common with various genetic backgrounds for improvement of seed dormancy through the use of marker-assisted selection. Further detailed research using near isogenic lines will be needed to define how this major QTL interacts with environmental conditions in our area.
Similar content being viewed by others
References
Anderson, J.A., M. Sorrells & S.D. Tanksley, 1993. RFLP analysis of genomic regions associated with resistance to pre-harvest sprouting in wheat. Crop Sci 33: 453–459.
Endo, T.R. & B.S. Gill, 1996. The deletion stocks of common wheat. J Hered 87: 295–307.
Flintham, J., R. Adlam, M. Bassoi, M. Holdsworth & M. Gale, 2002. Mapping genes for resistance to sprouting damage in wheat. Euphytica 126: 39–45.
Groos, C., G. Gay, M.R. Perretant, L. Gervais, M. Bernald, F. Dedryver & G. Charmet, 2002. Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theor Appl Genet 104: 39–47.
Gupta, P.K., H.S. Balyan, K.J. Edwards, P. Isaac, V. Korzun, M. Röder, M.F. Gautier, P. Joudrier, A.R. Schlatter, J. Dubcovsky, R.C. DelaPena, M. Khairallah, G. Penner, M.J. Hayden, P. Sharp, B. Keller, R.C.C. Wang, J.P. Hardouin, P. Jack & P. Leroy, 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105: 413–422.
Kato, K., W. Nakamura, T. Tabiki & H. Miura, 2001. Detection of loci controlling seed dormancy on group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 102: 980–985.
Mares, D.J. & K. Mrva, 2001. Mapping quantitative trait loci associated with variation in grain dormancy in Australian wheat. Aust J Agric Res 52: 1257–1265.
Noda, K., T. Matsuura, M. Maekawa & S. Taketa, 2002. Chromosome responsible for sensitivity of embryo to abscisic acid and dormancy in wheat. Euphytica 126: 203–209.
Osanai, S.I. & Y. Amano, 1993. Selection of tolerant lines to low temperature germinability in wheat. In: M.K. Walker-Simmons & J.L. Ried (Eds.), Proceedings of Sixth International Symposium on Pre-harvest Sprouting in Cereals, Westview Press, Boulder CO, USA, pp. 76–82.
Röder, M.S., V. Korzun, K. Wendehake, J. Plaschke, M.H. Tixer, P. Leroy & M.W. Ganal, 1998. A microsatellite map of wheat. Genetics 149: 2007–2023.
Roy, J.K., M. Prasad, R.K. Varshney, H.S. Balayan, T.K. Blake, H.S. Dhaliwal, H. Shingh, K.J. Edwards & P.K. Gupta, 1999. Identification of a microsatellite on chromosome 6B and a STS on 7D of bread wheat showing association with preharvest sprouting tolerance. Theor Appl Genet 99: 336–340.
Torada, A. & Y. Amano, 2002. Effect of seed coat color on seed dormancy in different environments. Euphytica 126: 99–105.
Torada, A. & M. Koike, 2004. Construction of SSR based genetic map with new markers using intraspecific population in hexaploid wheat. Plant Anim Genome XII, 173.
Zanetti, S., M. Winzeler, M. Keller & M. Messmer, 2000. Genetic analysis of pre-harvest sprouting resistance in a wheat × spelt cross. Crop Sci 40: 1406–1417.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Torada, A., Ikeguchi, S. & Koike, M. Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat. Euphytica 143, 251–255 (2005). https://doi.org/10.1007/s10681-005-7872-2
Issue Date:
DOI: https://doi.org/10.1007/s10681-005-7872-2