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Inhibition on brown rot disease and induction of defence response in harvested peach fruit by nitric oxide solution

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Abstract

Nitric oxide (NO) is an important signal molecule involved in numerous plant responses to biotic and abiotic stresses. The effect of nitric oxide (NO) solution on pathogen infection and defence response of peach (Prunus persica (L.) Batsch) fruit against brown rot disease caused by Monilinia fructicola was investigated. The results showed that 15 μmol l−1 NO solution did not significantly inhibit spore germination, germ tube length or pathogenicity of M. fructicola, but significantly reduced disease incidence and lesion areas in the fruit. Although 100 μmol l−1 NO solution effectively inhibited the spore germination, germ tube elongation and pathogenicity of M. fructicola, the high concentration of NO solution caused damage to the fruit. Moreover, 15 μmol l−1 NO enhanced the activities of chitinase (CHI) and β-1,3-glucanase (GNS) in the fruit. RT-PCR analysis showed that the expression of four genes, CHI, GNS, pathogenesis-related protein 1 and 10 genes (PR-1, PR-10) all increased after NO treatment. Conversely, pretreatment with 100 μmol l−1 NO scavenger, 2-4-carboxyphenyl-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), rendered the fruit relatively susceptible to pathogen infection and inhibited the defence response of the fruit. These results suggest that NO solution treatment can protect peach fruit from pathogen infection by inducing the activities of the defence enzymes and the expression of PR genes.

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Acknowledgments

The research work was supported by the Project of National Natural Science Foundation of China (31101371), Shandong Natural Science Foundation (ZR2010CQ039), the Research Fund for the Doctoral Program of Higher Education of China (20113702120001) and China Postdoctoral Science Foundation (201104605).

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Authors and Affiliations

  1. College of Food Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong Province, China

    Na Liu & Jingying Shi

  2. College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, Shandong Province, China

    Shuhua Zhu & Jie Zhou

  3. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu Province, China

    Rongxin Gu

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  1. Rongxin Gu
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Correspondence to Jingying Shi.

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Gu, R., Zhu, S., Zhou, J. et al. Inhibition on brown rot disease and induction of defence response in harvested peach fruit by nitric oxide solution. Eur J Plant Pathol 139, 369–378 (2014). https://doi.org/10.1007/s10658-014-0393-x

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