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Histopathology of durable adult plant resistance to leaf rust in the Brazilian wheat variety Toropi

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Abstract

Leaf rust, caused by the fungus Puccinia triticina is a major disease of wheat (Triticum aestivum) worldwide. This disease is prevalent in southern South America where the environmental conditions and high genetic variability of P. triticina favour epidemics. The primary means of controlling pathogenic P. triticina races has been through using wheat varieties containing race-specific resistance genes. The defence mechanisms involved in durable race non-specific resistance to P. triticina are probably distinct from those involved in non-durable race-specific resistance. We investigated the histological components of resistance to P. triticina present in three wheat genotypes: the race non-specific resistant Brazilian variety Toropi; the race-specific resistant line RL6010 Lr9; and the susceptible Brazilian variety BRS 194. Plants of these three genotypes were inoculated with P. triticina race MFP and tissue samples excised from flag leaves at various times after inoculation to assess the number of infective structures, frequency of cell death and the accumulation of autofluorescent cells and hydrogen peroxide. The genotypes showed different resistance mechanisms active at different times during the infection process. Our results for Toropi indicate that there was a reduction in the extent of formation of stomatal appressoria and all subsequent structures. During attempted penetration we also observed the production of autofluorescent compounds and late cell death, but not peroxide formation. This non-specific resistance to P. triticina involves both pre-haustorial and post-haustorial mechanisms which may be responsible for maintaining the low disease severity observed in this variety even under high inoculum pressure.

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Acknowledgments

The authors thank the Brazilian Federal Agency for the Coordination of the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES) for a scholarship to the first author and the Brazilian National Council for Scientific Research and Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq) and the Foundation for Research Support of Rio Grande do Sul State (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, FAPERGS) for financial support.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Fitotecnia, Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil

    Caroline Wesp-Guterres

  2. Departamento de Fitossanidade, Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil

    José Antônio Martinelli

  3. Universidade Estadual de Mato Grosso do Sul, 79200-000, Aquidauana, MS, Brazil

    Felipe André Sganzerla Graichen

  4. Embrapa—Centro Nacional de Pesquisa de Trigo, 99001-970, Passo Fundo, RS, Brazil

    Márcia Soares Chaves

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  1. Caroline Wesp-Guterres
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  3. Felipe André Sganzerla Graichen
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  4. Márcia Soares Chaves
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Correspondence to José Antônio Martinelli.

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Wesp-Guterres, C., Martinelli, J.A., Graichen, F.A.S. et al. Histopathology of durable adult plant resistance to leaf rust in the Brazilian wheat variety Toropi. Eur J Plant Pathol 137, 181–196 (2013). https://doi.org/10.1007/s10658-013-0232-5

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