Abstract
Cereal rusts are a constant disease threat that limits the production of almost all agricultural cereals. Rice is atypical in that it is an intensively grown agricultural cereal that is immune to rust pathogens. This immunity is manifested by nonhost resistance (NHR), the mechanisms of which are poorly understood. As part of the Borlaug Global Rust Initiative (BGRI), studies are being undertaken to dissect the molecular mechanisms that provide rust immunity in rice and determine if they can be transferred to wheat via transgenesis. Microscopic analyses showed that cereal rusts are capable of entering the rice leaf via formation of an appressorium over a stomate and subsequent infection of underlying mesophyll cells. However, there is considerable variation in the extent of colonization at each infection site. Our research effort has focused on screening for increased growth of cereal rust using natural and induced variants of rice. Two collections of rice mutants, T-DNA insertional mutants and chemical/irradiation-induced mutants, and diverse germplasm accessions are being screened for compromised NHR to cereal rusts. Preliminary screening with stripe rust identified several potential mutants that allow increased fungal growth. The confirmation of these lines will serve as the foundation for the isolation of gene(s) responsible for this compromised resistance. Details of the strategies being undertaken and progress to date are provided.
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References
Ayliffe M, Singh R, Lagudah E (2008) Durable resistance to wheat stem rust needed. Curr Opin Plant Biol 11:187–192
Ayliffe M, Jin Y, Steffenson B, Kang Z, Wang S, Leung H (2009) Molecular-genetic dissection of rice nonhost resistance to wheat stem rust. In: McIntosh RA (ed) Proceedings of the Borlaug Global Rust Initiative technical workshop, 17–20 Mar 2009. BGRI, Cd. Obregon, Mexico
Bednarek P, Pislewska-Bednarek M, Svatos A, Schneider B, Doubsky J, Mansurova M, Humphry M, Consonni C, Pangstruga R, Sanchez-Vallet A, Molina A, Schulze-Lefert P (2009) A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense. Science 323:101–106
Clay NK, Adio AM, Denoux C, Jander G, Ausubel FM (2009) Glucosinolate metabolites required for an Arabidopsis innate immune response. Science 323:95–101
Collins NC, Thordal-Christensen H, Lipka V, Bau S, Kombrink E, Qui JL, Huckelhoven R, Stein M, Freialdenhoven A, Somerville SC, Schulze-Lefert P (2003) SNARE-protein-mediated disease resistance at the plant cell wall. Nature 425:973–977
Hogenhout SA, van der Hoorn RAL, Terauchi R, Kamoun S (2009) Emerging concepts in effector biology of plant-associated organisms. Mol Plant Microbe Interact 22:115–122
Jafary H, Albertazzi G, Marcel TC, Niks RE (2008) High diversity of genes for nonhost resistance of barley to heterologous rust fungi. Genetics 178:2327–2339
Jones JDG, Dangl JL (2006) The plant immune system. Nature 444:323–329
Kim D-Y, Bovet L, Maeshima M, Martinoia E, Lee Y (2007) The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance. Plant J 50:207–218
Lipka V, Dittgen J, Bednarek P, Bhat R, Wiermer M, Stein M, Landtag J, Brandt W, Rosahl S, Scheel D, Llorente F, Molina A, Parker J, Somerville S, Schulze-Lefert P (2005) Pre- and postinvasion defenses both contribute to nonhost resistance in Arabidopsis. Science 310:1180–1183
Lipka U, Fuchs R, Lipka V (2008) Arabidopsis non-host resistance to powdery mildews. Curr Opin Plant Biol 11:404–411
Loehrer M, Langenbach C, Goellneer K, Conrath U, Schaffrath U (2008) Characterisation of nonhost resistance of Arabidopsis to the Asian soybean rust. Mol Plant Microbe Interact 21:1421–1430
Mellersch DG, Heath MC (2003) An investigation into the involvement of defense signalling pathways in components of the nonhost resistance of Arabidopsis to rust fungi also reveals a model system for studying rust fungal compatibility. Mol Plant Microbe Interact 16:398–404
Prats E, Martinez F, Rojas-Molina MM, Rubiales D (2007) Differential effects of phenylalanine ammonia lyase, cinnamyl alcohol dehydrogenase, and energetic metabolism inhibition on resistance of appropriate host and nonhost cereal–rust interactions. Phytopathology 97:1578–1583
Pretorius ZA, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis 84:203
Shafiei R, Hang C, Kang J-G, Loake GJ (2007) Identification of loci controlling non-host disease resistance in Arabidopsis against the leaf rust pathogen Puccinia triticina. Mol Plant Pathol 8:773–784
Singh RP, Hodson DP, Jin Y, Huerta-Espino J, Kinyua MG, Wanyera R, Njau P, Ward RW (2006) Current status, likely migration and strategies to mitigate the threat to wheat production from race Ug99 (TTKS) of stem rust pathogen. CAB Rev Perspect Agric Vet Sci Nutr Nat Resour 1:1–13
Singh RP, Kinyua MG, Wanyera R, Njau P, Jin Y, Huerta-Espino J (2007) Spread of a highly virulent race of Puccinia graminis tritici in eastern Africa: challenges and opportunities. In: Buck HT, Nisi E, Salomon N (eds) Developments in plant breeding, vol 12, wheat production in stressed environments. Springer, Netherlands
Stein M, Dittgen J, Sanchez-Rodriguez C, Hou B-H, Molina A, Schulze-Lefert P, Lipka V, Somerville S (2006) Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. Plant Cell 18:731–746
Stokstad E (2007) Deadly wheat fungus threatens world’s breadbaskets. Science 315:1786–1787
Wiermer M, Feys BJ, Parker JE (2005) Plant immunity: the EDS1 regulatory node. Curr Opin Plant Biol 8:383–389
Wu J, Wu C, Lei C, Baraoidan M, Boredos A, Madamba RS, Ramos-Pamplona M, Mauleon R, Portugal A, Ulat V, Bruskiewich R, Wang GL, Leach JE, Khush G, Leung H (2005) Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol Biol 59:85–97
Zhang J, Guo D, Chang Y, You C, Li X, Dai X, Weng Q, Zhang J, Chen G, Li X, Liu H, Han B, Zhang Q, Wu C (2007) Non-random distribution of T-DNA insertions at various levels of the genome hierarchy as revealed by analyzing 13 804 T-DNA flanking sequences from an enhancer-trap mutant library. Plant J 49:947–959
Zhao B, Lin X, Poland J, Trick H, Leach J, Hulbert S (2005) A maize resistance gene functions against bacterial streak disease in rice. Proc Natl Acad Sci USA 102:15383–15388
Zipfel C (2008) Pattern-recognition receptors in plant innate immunity. Curr Opin Immunol 20:10–16
Acknowledgments
We thank the Durable Rust Resistance in Wheat project led by Cornell University and supported by the Bill and Melinda Gates Foundation for financial support and Lachlan Lake of CSIRO and Suzette Madamba of IRRI for technical assistance.
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Ayliffe, M., Jin, Y., Kang, Z. et al. Determining the basis of nonhost resistance in rice to cereal rusts. Euphytica 179, 33–40 (2011). https://doi.org/10.1007/s10681-010-0280-2
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DOI: https://doi.org/10.1007/s10681-010-0280-2