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Characterization of mechanisms of resistance against Didymella pinodes in Pisum spp.

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Abstract

Ascochyta blight caused by Didymella pinodes is a serious disease of pea (Pisum sativum ssp. sativum) to which little resistance has been identified so far. Only incomplete resistance is available in pea germplasm although higher levels of resistance have been reported in related Pisum species. In this study we characterized histochemically the underlying resistance mechanisms in these wild species and in the pea cv. Radley, the pea cultivar with the highest level of resistance to D. pinodes. Resistance was characterized by a reduced success of colony establishment and lesion size. Histologically this was associated with higher frequency of epidermal cell death and protein cross-linking in infected epidermal cells but not with H2O2 accumulation and peroxidase activity.

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Acknowledgments

E. Carrillo was granted by a Cabildo de La Palma- CSIC PhD grant and S. Fondevilla by a contract funded by the Spanish JAEdoc program. Financial support by projects AGL2008-0239 and AGL2011-22524, cofinanced by FEDER, is acknowledge.

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Authors and Affiliations

  1. Institute for Sustainable Agriculture, CSIC, Avda. Menéndez Pidal S/N, Apdo. 4084, 14080, Córdoba, Spain

    E. Carrillo, D. Rubiales & S. Fondevilla

  2. IFAPA, Centro Alameda del Obispo, Avda. Menéndez Pidal S/N, Apdo. 3092, 14080, Córdoba, Spain

    A. Pérez-de-Luque

Authors
  1. E. Carrillo
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  2. D. Rubiales
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  3. A. Pérez-de-Luque
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  4. S. Fondevilla
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Correspondence to E. Carrillo.

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Carrillo, E., Rubiales, D., Pérez-de-Luque, A. et al. Characterization of mechanisms of resistance against Didymella pinodes in Pisum spp.. Eur J Plant Pathol 135, 761–769 (2013). https://doi.org/10.1007/s10658-012-0116-0

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  • DOI: https://doi.org/10.1007/s10658-012-0116-0

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