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Lactose-inducted production of a complete lignocellulolytic enzyme system by a novel bacterium Bacillus sp. BS-5 and its application for saccharification of alkali-pretreated corn cob

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Abstract

In this study, with combined carboxymethyl cellulose agar plate, xylan agar plate and filter paper hydrolysis assay, a novel cellulase and xylanase-producing strain identified as Bacillus sp. was isolated. Using lactose as the only carbon source, a complete and balanced lignocellulolytic enzyme system containing at least endoglucanase (9.6 U/ml), exoglucanase (0.8 U/ml), Fpase (1.4 U/ml), xylanase (3.8 U/ml) and β-glucosidase (1.2 U/ml) was produced. Interestingly, a zymogram of the crude culture supernatant displayed a multifunctional lignocellulolytic enzyme system including at least four bonds with both endoglucanase activity and xylanase activity at 21.2, 23.8, 28.9 and 31.2 kDa, respectively, indicating that these enzymes might be bifunctional. More gratifyingly, according to the binding affinity analysis and scanning electron microscopy, the crude enzyme complex produced by strain BS-5 was capable of hydrolyzing not only pure insoluble polysaccharides, but also agricultural residues such as corn cob. At 5% substrate concentration and 20 FPU/g enzyme loading, the reducing sugar was 350.8 mg/g of alkali-pretreated corn cob after 72 h enzymatic hydrolysis. These results suggested that this strain could be a good candidate for the development of a more cost-effective and efficient lignocellulolytic enzyme cocktail for the saccharification of lignocellulosic biomass.

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Acknowledgments

This research was financially supported by the Natural Science Foundation of Jiangsu (BK 20141456), the National Basic Research Program of the People’s Republic of China (2011CB707405) and the Natural Science Foundation of China (21406083).

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Authors and Affiliations

  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, Jiangsu, China

    Zhen Gao & Bin Wu

  2. Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, 223300, Jiangsu, China

    Jiaxing Xu

  3. Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing, 211816, Jiangsu, China

    Bin Wu & Bingfang He

  4. School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211816, Jiangsu, China

    Bingfang He

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  1. Jiaxing Xu
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  2. Zhen Gao
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  3. Bin Wu
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  4. Bingfang He
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Correspondence to Bin Wu.

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Xu, J., Gao, Z., Wu, B. et al. Lactose-inducted production of a complete lignocellulolytic enzyme system by a novel bacterium Bacillus sp. BS-5 and its application for saccharification of alkali-pretreated corn cob. Cellulose 24, 2059–2070 (2017). https://doi.org/10.1007/s10570-017-1247-4

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  • DOI: https://doi.org/10.1007/s10570-017-1247-4

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