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Effect of alginate matrix composition on regrowth of in vitro-derived encapsulated apical microcuttings of hybrid aspen

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Biologia Plantarum

Abstract

Effect of alginate matrix composition on regrowth performance of encapsulated microcuttings of hybrid aspen (Populus tremula L. × P. tremuloides Mincx.) was studied. Both high regrowth frequency and viability of explants were registered in all encapsulation mixtures tested. Some ingredients of the matrix (nutrient medium salts, sugars, growth regulators) significantly affected the initial development of the microcuttings. Sucrose appeared to play an important role in the starting stage of the regrowth event.

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Abbreviations

BAP:

6-benzylaminopurine

MS medium:

Murashige and Skoog medium

PGRs:

plant growth regulators

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Authors and Affiliations

  1. Tissue Culture Lab, Forest Research Institute, Kl. Ohridski Blvd. 132, BG-1756, Sofia, Bulgaria

    I. Tsvetkov

  2. Department of Environment and Agrobiotechnology, CRP Gabriel Lippmann, L-4422, Belvaux, Luxembourg

    L. Jouve & J. -F. Hausman

Authors
  1. I. Tsvetkov
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  2. L. Jouve
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  3. J. -F. Hausman
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Correspondence to I. Tsvetkov.

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Tsvetkov, I., Jouve, L. & Hausman, J.F. Effect of alginate matrix composition on regrowth of in vitro-derived encapsulated apical microcuttings of hybrid aspen. Biol Plant 50, 722–724 (2006). https://doi.org/10.1007/s10535-006-0115-0

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  • DOI: https://doi.org/10.1007/s10535-006-0115-0

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