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Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

  • Original Papers
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Biologia Plantarum

Abstract

The aim of this research was to determine whether exogenous abscisic acid (ABA) applied immediately after ex vitro transfer of in vitro grown plants can improve their acclimatization. Tobacco (Nicotiana tabacum L.) plantlets were transferred into pots with Perlite initially moistened either by water or 50 µM ABA solution and they were grown under low (LI) or high (HI) irradiance of 150 and 700 µmol m−2 s−1, respectively. Endogenous content of ABA in tobacco leaves increased considerably after ABA application and even more in plants grown under HI. Stomatal conductance, transpiration rate and net photosynthetic rate decreased considerably 1 d after ex vitro transfer and increased thereafter. The gas exchange parameters were further decreased by ABA application and so wilting of these plants was limited. Chlorophyll (a+b) and β-carotene contents were higher in ABA-treated plants, but the content of xanthophyll cycle pigments was not increased. However, the degree of xanthophyll cycle pigments deepoxidation was decreased what also suggested less stress in ABA-treated plants. No dramatic changes in most chlorophyll a fluorescence parameters after ex vitro transfer suggested that the plants did not suffer from restriction of electron transport or photosystem damage.

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Abbreviations

ABA:

abscisic acid

Car:

carotenoids

Chl:

chlorophyll

DEPS:

degreee of XCP deepoxidation

Fm :

maximum chlorophyll fluorescence

Fv :

variable chlorophyll fluorescence

PS:

photosystem

qNP :

non-photochemical quenching

qP :

photochemical quenching

RWC:

relative water content

WUE:

water use efficiency

XCP:

xanthophyll cycle pigments

ΦPS2 :

quantum yield of PS 2 photochemistry

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Authors and Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1, CZ-16000, Prague, Czech Republic

    J. Pospíšilová, H. Synková & D. Haisel

  2. Faculty of Science, Charles University in Prague, Vinièná 5, CZ-12844, Prague, Czech Republic

    P. Baťková

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  1. J. Pospíšilová
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  2. H. Synková
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  3. D. Haisel
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  4. P. Baťková
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Pospíšilová, J., Synková, H., Haisel, D. et al. Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets. Biol Plant 53, 11–20 (2009). https://doi.org/10.1007/s10535-009-0003-5

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