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Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes

  • Published:
Biologia Plantarum

Abstract

Abscisic acid (ABA) and salicylic acid (SA) were sprayed on leaves of wheat genotypes C 306 and Hira at 25 and 40 d after sowing under moderate water stress (−0.8 MPa) imposed by adding PEG-6000 in nutrient solution. ABA and SA increased the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and catalase in comparison to unsprayed control plants. Both ABA and SA treatments decreased the contents of hydrogen peroxide and thiobarbituric acid reactive substances, a measure of lipid peroxidation, compared to unsprayed plants. The beneficial effect of increase in antioxidant enzymes activity and decrease in oxidative stress was reflected in increase in chlorophyll and carotenoid contents, relative water content, membrane stability index, leaf area and total biomass over control plants. The lower concentrations of ABA (0.5 mM) and SA (1.0 mM) were generally more effective than higher concentrations.

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Abbreviations

ABA:

abscisic acid

APOX:

ascorbate peroxidase

Car:

carotenoids

CAT:

catalase

Chl:

chlorophyll

DAS:

days after sowing

DMSO:

dimethylsulfoxide

DTNB:

5,5-dithiobis-(2-nitrobenzoic acid)

EDTA:

ethylene diamine tetraacetic acid sodium salt

GR:

glutathione reductase

GSSG:

oxidized glutathione

MSI:

membrane stability index

NBT:

nitrobluetetrazolium chloride

ROS:

reactive oxygen species

SA:

salicylic acid

SOD:

superoxide dismutase

TBARS:

thiobarbituric acid reactive substances

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    S. Agarwal, R. K. Sairam, G. C. Srivastava & R. C. Meena

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  1. S. Agarwal
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  2. R. K. Sairam
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  3. G. C. Srivastava
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  4. R. C. Meena
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Correspondence to R. K. Sairam.

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Agarwal, S., Sairam, R.K., Srivastava, G.C. et al. Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biol Plant 49, 541–550 (2005). https://doi.org/10.1007/s10535-005-0048-z

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  • DOI: https://doi.org/10.1007/s10535-005-0048-z

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