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Involvement of exopeptidases in dehydration tolerance of spring wheat seedlings

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Biologia Plantarum

Abstract

The observed inability of 6-d-old seedlings of spring wheat (Triticum aestivum L.) to tolerate the same water deficit as compared to the 4-d-old seedlings seems to be associated with the higher carboxypeptidase and lower aminopeptidase activities. Free amino acid pools differentiated also the 4-d-old seedlings from the older ones. Dehydration decreased the amino acid content in 4-d-old seedlings, increased it in 6-d-old seedlings and changed composition of amino acid pool. In tolerant phase of wheat seedling growth carboxypeptidase activity increased in response to water deficit and aminopeptidase activity increased in dehydrated seedlings, independently of their age.

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Abbreviations

Ala:

alanine

Arg:

arginine

Asn:

asparagine

Asp:

aspartic acid

β-NA:

β-naphthylamide

Cys:

cysteine

DMSO:

dimethyl sulfoxide

EDTA:

ethylendiaminetetraacetic acid disodium salt

E64:

(2S,3S)-3-(N-{(S)-1-[N-(4-guanidinobutyl)carbamoyl]3-methylbutyl}carbamoyl)oxirane-2-carboxylic acid

HPLC:

high pressure liquid chromatography

Gly:

glycine

Gln:

glutamic acid

Glu:

glutamine

Ile:

isoleucine

Leu:

leucine

Lys:

lysine

Met:

methionine

PAGE:

polyacrylamide gel electrophoresis

Phe:

phenylalanine

pCMB:

p-chloromercuribenzoate

PMSF:

phenylmethylsulfonyl fluoride

Pro:

proline

PVP:

polyvinylpolypyrrolidone

RWC:

relative water content

Ser:

serine

Thr:

threonine

TNBS:

trinitrobenzene sulfonate

Trp:

tryptophan

Tyr:

tyrosine

Val:

valine

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Authors and Affiliations

  1. Biochemistry Department, Warsaw University of Life Sciences, Nowoursynowska 159, PL-02776, Warszawa, Poland

    A. Miazek & B. Zagdańska

  2. Plant Physiology and Biochemistry Department, Plant Breeding and Acclimatization Institute, Radzików, POB 1019, PL-00850, Warszawa, Poland

    B. Zagdańska

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  1. A. Miazek
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  2. B. Zagdańska
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Correspondence to B. Zagdańska.

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Miazek, A., Zagdańska, B. Involvement of exopeptidases in dehydration tolerance of spring wheat seedlings. Biol Plant 52, 687–694 (2008). https://doi.org/10.1007/s10535-008-0133-1

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  • DOI: https://doi.org/10.1007/s10535-008-0133-1

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