Abstract
Following drought stress at supraoptimal temperature the increase in proline (Pro) content in transgenic (T) soybean [Glycine max (L.) Merr. cv. Ibis] plants overexpressing the gene coding for the last enzyme of Pro biosynthesis, L-Δ1-pyrroline-5-carboxylate reductase, was much greater than in wild type (W) plants (105-fold versus 19-fold after 7 d). Under control conditions arginine accounted for nearly 60 % of the total free amino acid content. After stress treatment the content of Pro was more than 50 % in both T and W genotypes, and at the end of recovery the γ-aminobutyrate content reached 27 and 53 % in the W and T plants, respectively. Without stress treatment there was only a 2-fold difference between T and W in the tyrosine content. However, during the stress period and the subsequent recovery a similar difference was found for many amino acids. The present results indicate that manipulating of the content of a single amino acid influences the whole free amino acid composition in soybean.
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Abbreviations
- Arg:
-
arginine
- Asn:
-
asparagine
- Asp:
-
aspartate
- DM:
-
dry mass
- Gaba:
-
γ-aminobutyrate
- Gln:
-
glutamine
- Glu:
-
glutamate
- His:
-
histidine
- Ile:
-
isoleucine
- Leu:
-
leucine
- Phe:
-
phenylalanine
- P5CR:
-
L-Δ1-pyrroline-5-carboxylate reductase
- Pro:
-
proline
- PS:
-
preliminary stress
- RWC:
-
relative water content
- Ser:
-
serine
- T:
-
transgenic plants
- Tyr:
-
tyrosine
- W:
-
wild type
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Simon-Sarkadi, L., Kocsy, G., Várhegyi, Á. et al. Stress-induced changes in the free amino acid composition in transgenic soybean plants having increased proline content. Biol Plant 50, 793–796 (2006). https://doi.org/10.1007/s10535-006-0134-x
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DOI: https://doi.org/10.1007/s10535-006-0134-x