Abstract
Osmotic stress induced with 1 M sorbitol inhibited δ-aminolevulinic acid dehydratase (ALAD) and aminolevulinic acid (ALA) synthesizing activities in etiolated maize leaf segments during greening; the ALAD activity was inhibited to a greater extent than the ALA synthesis. When the leaves were exposed to light, the ALAD activity increased for the first 8 h, followed by a decrease observed at 16 and 24 h in both sorbitol-treated and untreated leaf tissues. The maximum inhibition of the enzyme activity was observed in the leaf segments incubated with sorbitol for 4 to 8 h. Glutamate increased the ALAD activity in the in vitro enzymatic preparations obtained from the sorbitol-treated leaf segments; sorbitol inhibited the ALAD activity in the preparations from both sorbitol-treated and untreated leaves. It was suggested that sorbitol-induced osmotic stress inhibits the enzyme activity by affecting the ALAD induction during greening and regulating the ALAD steady-state level of ALAD in leaf cells. The protective effect of glutamate on ALAD in the preparations from the sorbitol-treated leaves might be due to its stimulatory effect on the enzyme.
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Abbreviations
- ALA:
-
5-aminolevulinic acid
- ALAD:
-
d-aminolevulinic acid dehydratase
- GluTR:
-
glutamyl-tRNA reductase
- GSAT:
-
glutamate semialdehyde amino transferase
- PBG:
-
porphobilinogen
- PBGD:
-
porphobilinogen deaminase
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Published in Russian in Biokhimiya, 2018, Vol. 83, No. 1, pp. 95-100.
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Jain, M., Tiwary, S. & Gadre, R. Modulation of δ-aminolevulinic acid dehydratase activity by the sorbitol-induced osmotic stress in maize leaf segments. Biochemistry Moscow 83, 32–36 (2018). https://doi.org/10.1134/S0006297918010042
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DOI: https://doi.org/10.1134/S0006297918010042