Abstract
Corncob was treated by dilute H2SO4. The hydrolysate contained 45.7 g sugar/l. Without concentration or adding other nutrients, the hydrolysate, after being detoxified by overliming and adsorption with activated charcoal, was used for oil production using Trichosporon cutaneum. After 8 days’ growth in shake-flasks, the biomass was 22.1 g/l with a lipid content of 36%. The lipid yield per mass of sugar was 17.4% (w/w). Corncob thus is a promising raw material for microbial oil production by this yeast.
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References
Adamczak M, Bornscheuer UT, Bednarski W (2009) The application of biotechnological methods for the synthesis of biodiesel. Eur J Lipid Sci Technol 111:808–813
Cassales A, Souza-Cruz PB, Rech R et al (2011) Optimization of soybean hull acid hydrolysis and its characterization as a potential substrate for bioprocessing. Biomass Bioenergy 35:4675–4683
Chen X, Li Z, Zhang X, Hu F, Ryu D, Bao J (2009) Screening of oleaginous yeast strains tolerant to lignocellulose degradation compounds. Appl Biochem Biotechnol 159:1–14
Economou CN, Aggelis G, Pavlou S, Vayenas D (2011) Single cell oil production from rice hulls hydrolysate. Bioresour Technol 102:9737–9742
Heredia L, Ratledge C (1988) Simultaneous utilization of glucose and xylose by Candida curvata D in continuous culture. Biotechnol Lett 10:25–30
Huang C, Zong MH, Wu H, Liu QP (2009) Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour Technol 100:4535–4538
Huang C, Wu H, Li R, Zong MH (2012) Improving lipid production from bagasse hydrolysate with Trichosporon fermentans by response surface methodology. N Biotechnol 29:372–378
Li Q, Du W, Liu DH (2008) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80:749–756
Ratledge C (2004) Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie 86:807–815
Tsigie YA, Wang CY, Truong CT, Ju YH (2011) Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate. Bioresour Technol 102:9216–9222
Xue FY, Miao JX, Zhang X, Luo H, Tan TW (2008) Studies on lipid production by Rhodotorula glutinis fermentation using monosodium glutamate wastewater as culture medium. Bioresour Technol 99:5923–5927
Yu X, Zheng Y, Dorgan KM, Chen S (2011) Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. Bioresour Technol 102:6134–6140
Zhu LY, Zong MH, Wu H (2008) Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Bioresour Technol 99:7881–7885
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The authors acknowledge the financial support of industrialization project of high-new technology of Guangdong province (2009B011200008) and the important project of knowledge innovation engineering of Chinese Academy of Sciences (KSCX2-YW-G-063).
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Chen, Xf., Huang, C., Xiong, L. et al. Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum . Biotechnol Lett 34, 1025–1028 (2012). https://doi.org/10.1007/s10529-012-0869-8
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DOI: https://doi.org/10.1007/s10529-012-0869-8