Abstract
Lactobacillus helveticus, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg−1 of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of androst-4-ene-3, 17 dione within 48 h at 37°C with extracellular cholesterol oxidase activity at 12 U mg−1 and intracellular oxidase at 0.5 U mg−1.
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Acknowledgments
Bhushan Chaudhari gratefully acknowledges financial support and Jayesh Ahire is thankful to Research Fellowship in Science for Meritorious Students (RFSMS) from University Grants Commission, New Delhi.
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Ahire, J.J., Bhat, A.A., Thakare, J.M. et al. Cholesterol assimilation and biotransformation by Lactobacillus helveticus . Biotechnol Lett 34, 103–107 (2012). https://doi.org/10.1007/s10529-011-0733-2
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DOI: https://doi.org/10.1007/s10529-011-0733-2