Abstract
Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM≈1 g l−1) where the bacterium was grown for 24 h, at 30°C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg−1). The growing cells produced 0.076 g ADD from 1 g cholesterol l−1.
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Chaudhari, P.N., Chaudhari, B.L. & Chincholkar, S.B. Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum . Biotechnol Lett 32, 695–699 (2010). https://doi.org/10.1007/s10529-010-0206-z
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DOI: https://doi.org/10.1007/s10529-010-0206-z