Abstract
A strain of Bacillus coagulans that converted mixed sugars of glucose, xylose, and arabinose to l-lactic acid with 85% yield at 50°C was isolated from composted dairy manure. The strain was tolerant to aldehyde growth inhibitors at 2.5 g furfural/l, 2.5 g 5-hydroxymethylfurfural/l, 2.5 g vanillin/l, and 1.2 g p-hydroxybenzaldehyde/l. In a simultaneous saccharification and fermentation process, the strain converted a dilute-acid hydrolysate of 100 g corn fiber/l to 39 g lactic acid/l in 72 h at 50°C. Because of its inhibitor tolerance and ability to fully utilize pentose sugars, this strain has potential to be developed as a biocatalyst for the conversion of agricultural residues into valuable chemicals.
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Acknowledgments
Dairy manure compost was kindly provided by Dr. Thomas Edrington, USDA-ARS Southern Plain Agricultural Research Center, College Station, TX. The authors wish to thank Eric Hoecker, Imran Khan, and Jacqueline Zane for technical assistance.
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Bischoff, K.M., Liu, S., Hughes, S.R. et al. Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile Bacillus coagulans . Biotechnol Lett 32, 823–828 (2010). https://doi.org/10.1007/s10529-010-0222-z
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DOI: https://doi.org/10.1007/s10529-010-0222-z