Abstract
A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombinant virus was obtained from the hemolymph of injected larvae and the hemolymph then re-injected into the larvae as an inoculum. This resulted in a high-level production of foreign protein in the silkworm larvae. This technique produces easy and rapid recombinant protein production in silkworms.
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Acknowledgements
This work was supported by a grant from the Biogreen 21 Program, Rural Development Administration, Republic of Korea and the Brain Korea 21 Project, the Ministry of Education, Republic of Korea.
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Lee, K.S., Kim, B.Y., Je, Y.H. et al. A new technique for producing recombinant baculovirus directly in silkworm larvae. Biotechnol Lett 29, 175–180 (2007). https://doi.org/10.1007/s10529-006-9215-3
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DOI: https://doi.org/10.1007/s10529-006-9215-3