Abstract
The expression efficiency of an insect-derived cellulase was assayed in silkworm larvae infected with recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) mutants lacking the virus-encoded chitinase (chiA) and/or cathepsin (v-cath) genes. Expression was increased by approx. 10% in mutants lacking chiA or v-cath and 17% in a mutant lacking both chiA and v-cath compared with that of the unmodified recombinant BmNPV. The recombinant BmNPV lacking both chiA and v-cath can therefore be used for a large-scale production of foreign proteins in silkworms.
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Revisions requested 27 September 2005 and 16 December 2005; Revisions received 6 December 2005 and 3 February 2006
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Lee, K.S., Je, Y.H., Woo, S.D. et al. Production of a Cellulase in Silkworm Larvae using a Recombinant Bombyx mori nucleopolyhedrovirus lacking the Virus-encoded Chitinase and Cathepsin Genes. Biotechnol Lett 28, 645–650 (2006). https://doi.org/10.1007/s10529-006-0030-7
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DOI: https://doi.org/10.1007/s10529-006-0030-7