Skip to main content
SpringerLink
Log in

Genetic Characterization of Green Bean (Phaseolus vulgaris L.) Accessions from Turkey with SCAR and SSR Markers

  • Original Article
  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

Characterization, conservation, and utilization of genetic resources is essential for the sustainability in agriculture. Plant genetic resources are important for breeding efforts designed for the generation of new cultivars or for the improvement of existing ones. Green bean has been cultivated extensively in Turkey giving rise to local accessions through selection over time and adaptation to various environmental conditions. The objective of the present study was to determine the genetic relationships of green bean accessions collected from Kırşehir Province of Turkey, located at the central Anatolia. Within a population of 275 green bean accessions, 50 accessions were selected on the basis of morphological observations for further evaluation with SSR and STS/SCAR markers together with 4 reference cultivars of Andean and Mesoamerican origin. SSR markers selected on the basis of high polymorphism information content revealed the genetic relatedness of selected green bean accessions. STS/SCAR markers associated with bean anthracnose, common bacterial blight, white mold, halo blight, and phaseolin protein demonstrated the inheritance of resistance traits of local accessions at the selected loci. These findings may help better utilize genetic resources and furthermore are expected to facilitate forthcoming breeding studies for the generation of novel cultivars well adapted to the region.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Asfaw A, Blair MW, Almekinders C (2009) Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands. TAG 120(1):1–12

    Article  PubMed  Google Scholar 

  • Awale HE, Kelly JD (2001) Development of SCAR markers linked to Co-42 gene in common bean. Annu Rept Bean Improv Coop 44:119–120

    Google Scholar 

  • Bajpai PK, Warghat AR, Sharma RK, Yadav A, Thakur AK, Srivastava RB, Stobdan T (2014) Structure and genetic diversity of natural populations of Morus alba in the Trans-Himalayan Ladakh Region. Biochem Genet 52(3):137–152

    Article  CAS  PubMed  Google Scholar 

  • Balkaya A (1999) A research on collection of genetic resources, determination of phenological and morphological characteristics and selection of suitable types for fresh consumption by pedigree selection in green bean (Phaseolus vulgaris L.) in the Black Sea region. Dissertation, Samsun Ondokuz Mayıs University, Samsun, Turkey

  • Belletti P, Lotito S (1996) Identification of runner bean genotypes (Phaseolus coccineus L.) by isozyme analysis. J Genet Breed 50:185–190

    CAS  Google Scholar 

  • Bitocchi E, Bellucci E, Giardini A, Rau D, Rodriguez M, Biagetti E, Papa R (2013) Molecular analysis of the parallel domestication of the common bean (Phaseolus vulgaris) in Mesoamerica and the Andes. New Phytol 197(1):300–313

    Article  CAS  PubMed  Google Scholar 

  • Blair MW, Pedraza F, Buendia HF, Gaitán-Solís E, Beebe SE, Gepts P, Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). TAG 107(8):1362–1374

    Article  CAS  PubMed  Google Scholar 

  • Blair MW, Torres MM, Giraldo MC, Pedraza F (2009) Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.). BMC Plant Biol 9(1):100

    Article  PubMed  PubMed Central  Google Scholar 

  • Bowcock AM, Ruiz-Linares A, Tomfohrde J, Minch E, Kidd JR, Cavalli-Sforza LL (1994) High resolution of human evolutionary trees with polymorphic microsatellites. Nature 368(6470):455–457

    Article  CAS  PubMed  Google Scholar 

  • Burle ML, Fonseca JR, Kami JA, Gepts P (2010) Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity. TAG 121(5):801–813

    Article  PubMed  PubMed Central  Google Scholar 

  • Ceylan A, Öcal N, Akbulut M (2014) Genetic diversity among the Turkish common bean cultivars (Phaseolus vulgaris L.) as assessed by SRAP, POGP and cpSSR markers. Biochem Syst Ecol 54:219–229

    Article  CAS  Google Scholar 

  • Cortés AJ, Chavarro MC, Blair MW (2011) SNP marker diversity in common bean (Phaseolus vulgaris L.). TAG 123(5):827–845

    Article  PubMed  Google Scholar 

  • Durán LA, Blair MW, Giraldo MC, Macchiavelli R, Prophete E, Nin JC, Beaver JS (2005) Morphological and molecular characterization of common bean landraces and cultivars from the Caribbean. Crop Sci 45:1320–1328

    Article  Google Scholar 

  • FAO (2013) http://faostat.fao.org/

  • Feng SG, Lu JJ, Gao L, Liu JJ, Wang HZ (2014) Molecular phylogeny analysis and species identification of Dendrobium (Orchidaceae) in China. Biochem Genet 52(3):127–136

    Article  CAS  PubMed  Google Scholar 

  • Fourie D, Herselman I (2011) Application of molecular markers in breeding for bean common blight resistance in South Africa. Afr Crop Sci J 19(4):369–376

    Google Scholar 

  • Fourie D, Miklas PN, Ariyarathne HM (2004) Genes conditioning halo blight resistance to races 1, 7, and 9 occur in a tight cluster. Annu Rep Bean Improv Coop 47:103–104

    Google Scholar 

  • Gepts P (1998) Origin and evolution of common bean: past events and recent trends. HortSci 33:1124–1130

    Google Scholar 

  • Kaczmarska E, Gawronski J, Dyduch-Sieminska M, Najda A, Marecki W, Zebrowska J (2015) Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea). Turk J Agric For 39:394–402

    Article  Google Scholar 

  • Kami J, Velásquez VB, Debouck DG, Gepts P (1995) Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris. Proc Natl Acad Sci 92(4):1101–1104

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Keller B, Manzanares C, Jara C, Lobaton JD, Studer B, Raatz B (2015) Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.). TAG 128(5):813–826

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kelly JD, Vallejo VA (2004) A comprehensive review of the major genes conditioning resistance to anthracnose in common bean. HortSci 39(6):1196–1207

    CAS  Google Scholar 

  • Khaidizar MI, Haliloglu K, Elkoca E, Aydin M, Kantar F (2012) Genetic diversity of common bean (Phaseolus vulgaris L.) landraces grown in northeast Anatolia of Turkey assessed with simple sequence repeat markers. Turk J Field Crops 17(2):145–150

    Google Scholar 

  • Kumar V, Sharma S, Kero S, Sharma S, Sharma AK, Kumar M, Bhat KV (2008) Assessment of genetic diversity in common bean (Phaseolus vulgaris L.) germplasm using amplified fragment length polymorphism (AFLP). Sci Hortic 116:138–143

    Article  CAS  Google Scholar 

  • Kwak M, Gepts P (2009) Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae). TAG 118(5):979–992

    Article  CAS  PubMed  Google Scholar 

  • Lioi L, Piergiovanni AR, Pignone D, Puglisi S, Santantonio M, Sonnante G (2005) Genetic diversity of some surviving on-farm Italian common bean (Phaseolus vulgaris L.) landraces. Plant Breed 124(6):576–581

    Article  CAS  Google Scholar 

  • Madakbaş SH, Hız MC, Küçükyan S, Sayar MT (2013) Transfer of Co-1 gene locus for anthracnose disease resistance to fresh bean (Phaseolus vulgaris L.) through hybridization and molecular marker-assisted selection (MAS). J Agric Sci 5(4):94–100

    Google Scholar 

  • Meza N, Rosas JC, Martín JP, Ortiz JM (2013) Biodiversity of common bean (Phaseolus vulgaris L.) in Honduras, evidenced by morphological characterization. Genet Resour Crop Evoult 60(4):1329–1336

    Article  Google Scholar 

  • Miklas PN (2007) Marker-assisted backcrossing QTL for partial resistance to Sclerotinia white mold in dry bean. Crop Sci 47:935–942

    Article  CAS  Google Scholar 

  • Miklas PN, Smith JR, Riley R, Grafton KF, Singh SP, Jung G, Coyne DP (2000) Marker-assisted breeding for pyramided resistance to common bacterial blight in common bean. Ann Rep Bean Improv Coop 43:39–40

    Google Scholar 

  • Miklas PN, Fourie D, Trapp J, Davis J, Myers JR (2014) New loci including conferring resistance to halo bacterial blight on chromosome Pv04 in common bean. Crop Sci 54(5):2099–2108

    Article  Google Scholar 

  • Minch E, Ruiz-Linares A, Goldstein D, Feldman M, Cavalli-Sforza LL (1995) Microsat (version 1.5): A computer program for calculating various statistics on microsatellite allele data. Stanford University Medical Center, Stanford

  • Mishra PK, Ram RB, Kumar N (2015) Genetic variability, heritability, and genetic advance in strawberry (Fragaria × ananassa Duch.). Turk J Agric For 39:451–458

    Article  Google Scholar 

  • Paetkau D, Calvert W, Stirling I, Strobeck C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354

    Article  CAS  PubMed  Google Scholar 

  • Raggi L, Tiranti B, Negri V (2013) Italian common bean landraces: diversity and population structure. Genet Resour Crop Evoult 60(4):1515–1530

    Article  Google Scholar 

  • Rohlf FJ (1988) NTSYS-PC numerical taxonomy and multivariate analysis system. Exeter Publishing, New York

    Google Scholar 

  • Sarıkamış G, Yaşar F, Bakır M, Kazan K, Ergül A (2009) Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey. Genet Mol Res 8(3):880–887

    Article  PubMed  Google Scholar 

  • Sharma PN, Diaz LM, Blair MW (2013) Genetic diversity of two Indian common bean germplasm collections based on morphological and microsatellite markers. Plant Genet Resour 11(2):121–130

    Article  CAS  Google Scholar 

  • Sneath PH, Sokal RR (1973) Numerical taxonomy. Freeman, San Francisco

    Google Scholar 

  • Song Q, Jia G, Hyten DL, Jenkins J, Hwang EY, Schroeder SG, Osorno JM, Schmutz J, Jackson SA, McClean PE, Cregan PB (2015) SNP Assay Development for linkage map construction, anchoring whole-genome sequence, and other genetic and genomic applications in common bean. G3: Genes| Genomes| Genet 5(11):2285–2290

    Article  PubMed  PubMed Central  Google Scholar 

  • Wagner HW, Sefc KM (1999) Identity 1.0. Centre for Applied Genetics. University of Agricultural Science, Vienna

  • Yu K, Park SJ, Poysa V, Gepts P (2000) Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). J Hered 91:429–434

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study is dedicated to Assist. Prof. Dr. Seher Yıldız Madakbaş, who was the Project leader however has passed away in 2014. This project was funded by Ahi Evran University Project No. PYO-Fen.4001.12.001. Molecular analysis (SSR and SCAR marker analysis) was performed at Ankara University Faculty of Agriculture Department of Horticulture. SSR markers included in the project belong to a previous work. The fragment analysis was performed at Ankara University, Biotechnology Institute.

Author information

Authors and Affiliations

  1. Department of Horticulture, Faculty of Agriculture, Ahi Evran University, Kırşehir, Turkey

    Seher Yıldız Madakbaş & Hakan Başak

  2. Department of Horticulture, Faculty of Agriculture, Ankara University, 06110, Ankara, Turkey

    Gölge Sarıkamış

  3. Department of Animal Science, Faculty of Agriculture, Ahi Evran University, Kırşehir, Turkey

    Ufuk Karadavut

  4. Biotechnology Institute, Ankara University, Ankara, Turkey

    Canan Yüksel Özmen & Selin Çayan

  5. Ministry of Food, Agriculture and Livestock, Kırşehir, Turkey

    Mete Gürhan Daşçı

Authors
  1. Seher Yıldız Madakbaş
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gölge Sarıkamış
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hakan Başak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ufuk Karadavut
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Canan Yüksel Özmen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mete Gürhan Daşçı
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Selin Çayan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gölge Sarıkamış.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Madakbaş, S.Y., Sarıkamış, G., Başak, H. et al. Genetic Characterization of Green Bean (Phaseolus vulgaris L.) Accessions from Turkey with SCAR and SSR Markers. Biochem Genet 54, 495–505 (2016). https://doi.org/10.1007/s10528-016-9737-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-016-9737-x

Keywords

Search

Navigation