Skip to main content
SpringerLink
Log in

Molecular Phylogeny Analysis and Species Identification of Dendrobium (Orchidaceae) in China

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

Dendrobium plants are important commercial herbs in China, widely used in traditional medicine and ornamental horticulture. In this study, sequence-related amplified polymorphism (SRAP) markers were applied to molecular phylogeny analysis and species identification of 31 Chinese Dendrobium species. Fourteen SRAP primer pairs produced 727 loci, 97% of which (706) showed polymorphism. Average polymorphism information content of the SRAP pairs was 0.987 (0.982–0.991), showing that plenty of genetic diversity exists at the interspecies level of Chinese Dendrobium. The molecular phylogeny analysis (UPGMA) grouped the 31 Dendrobium species into six clusters. We obtained 18 species-specific markers, which can be used to identify 10 of the 31 species. Our results indicate the SRAP marker system is informative and would facilitate further application in germplasm appraisal, evolution, and genetic diversity studies in the genus Dendrobium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Aneja B, Yadav N, Chawla V, Yadav R (2012) Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement. Mol Breed 30(4):1635–1648

    Article  CAS  Google Scholar 

  • Bao X, Shun Q, Chen L (2001) The medicinal plants of Dendrobium (Shi-hu) in China. Shanghai Medicinal University Press and Fudan University Press, Shanghai

    Google Scholar 

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32(3):314–331

    CAS  PubMed Central  PubMed  Google Scholar 

  • Budak H, Shearman RC, Parmaksiz I, Gaussoin RE, Riordan TP, Dweikat I (2004) Molecular characterization of Buffalo grass germplasm using sequence-related amplified polymorphism markers. Theor Appl Genet 108(2):328–334

    Article  CAS  PubMed  Google Scholar 

  • Cai XY, Feng ZY, Zhang XX, Xu W, Hou BW, Ding XY (2011) Genetic diversity and population structure of an endangered Orchid (Dendrobium loddigesii Rolfe) from China revealed by SRAP markers. Sci Hortic 129(4):877–881

    Article  Google Scholar 

  • Carlsward BS, Stern WL, Judd WS, Lucansky TW (1997) Comparative leaf anatomy and systematics in Dendrobium, sections Aporum and Rhizobium (Orchidaceae). Int J Plant Sci 158(3):332–342

    Article  Google Scholar 

  • Ding G, Zhang DZ, Ding XY, Zhou Q, Zhang WC, Li XX (2008) Genetic variation and conservation of the endangered Chinese endemic herb Dendrobium officinale based on SRAP analysis. Plant Syst Evol 276(3–4):149–156

    Article  Google Scholar 

  • Ferriol M, Pico B, Nuez F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor Appl Genet 107(2):271–282

    Article  CAS  PubMed  Google Scholar 

  • Ferriol M, Pico B, de P, Nuez F (2004) Molecular diversity of a germplasm collection of squash (Cucurbita moschata) determined by SRAP and AFLP markers. Crop Sci 4:653–664

    Google Scholar 

  • Huang LK, Bughrara S, Zhang XQ, Bales-Arcelo C, Bin X (2011) Genetic diversity of switchgrass and its relative species in Panicum genus using molecular markers. Biochem Syst Ecol 39(4):685–693

    Article  CAS  Google Scholar 

  • Lau DTW, Shaw PC, Wang J, But PPH (2001) Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA. Planta Med 67(5):456–460

    Article  CAS  PubMed  Google Scholar 

  • Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103(2–3):455–461

    Article  CAS  Google Scholar 

  • Lu JJ, Wang S, Zhao HY, Liu JJ, Wang HZ (2012a) Genetic linkage map of EST-SSR and SRAP markers in the endangered Chinese endemic herb Dendrobium (Orchidaceae). Genet Mol Res 11(4):4654–4667

    Article  CAS  PubMed  Google Scholar 

  • Lu JJ, Zhao HY, Suo NN, Wang S, Shen B, Wang HZ, Liu JJ (2012b) Genetic linkage maps of Dendrobium moniliforme and D. officinale based on EST-SSR, SRAP, ISSR and RAPD markers. Sci Hortic 137:1–10

    Article  CAS  Google Scholar 

  • Morris MW, Stern WL, Judd WS (1996) Vegetative anatomy and systematics of subtribe Dendrobiinae (Orchidaceae). Bot J Linn Soc 120(2):89–144

    Article  Google Scholar 

  • Mutlu N, Boyaci FH, Gocmen M, Abak K (2008) Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant. Theor Appl Genet 117(8):1303–1312

    Article  CAS  PubMed  Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76(10):5269–5273

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Rahman M, McVetty PB, Li G (2007) Development of SRAP, SNP and multiplexed SCAR molecular markers for the major seed coat color gene in Brassica rapa L. Theor Appl Genet 115(8):1101–1107

    Article  CAS  PubMed  Google Scholar 

  • Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5(2):69–76

    Article  CAS  PubMed  Google Scholar 

  • Rohlf F (1997) NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.2. Applied Biostatistics, New York

  • Stern WL, Morris MW, Judd WS (1994) Anatomy of the thick leaves in Dendrobium section Rhizobium (Orchidaceae). Int J Plant Sci 155(6):716–729

    Article  Google Scholar 

  • Suman A, Kimbeng CA, Edmé SJ, Veremis J (2008) Sequence-related amplified polymorphism (SRAP) markers for assessing genetic relationships and diversity in sugarcane germplasm collections. Plant Genet Res 6(03):222

    Article  Google Scholar 

  • Tsi ZH, Chen SC, Luo YB, Zhu GH (1999) Orchidaceae (3). Flora Republicae Popularis Sinicae. Science Press, Beijing

  • Wang H-Z, Feng S-G, Lu J-J, Shi N-N, Liu J-J (2009) Phylogenetic study and molecular identification of 31 Dendrobium species using inter-simple sequence repeat (ISSR) markers. Sci Hortic 122(3):440–447

    Article  CAS  Google Scholar 

  • Weir BS (1990) Genetic data analysis. Methods for discrete population genetic data. Sinauer Associates, Sunderland, p 377

    Google Scholar 

  • Xue D, Feng S, Zhao H, Jiang H, Shen B, Shi N, Lu J, Liu J, Wang H (2010) The linkage maps of Dendrobium species based on RAPD and SRAP markers. J Genet Genomics (Yi chuan xue bao) 37(3):197–204

    Article  CAS  Google Scholar 

  • Yue GH, Lam-Chan LT, Hong Y (2006) Development of simple sequence repeat (SSR) markers and their use in identification of Dendrobium varieties. Mol Ecol Notes 6(3):832–834

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was funded in part by the National Natural Science Foundation (No. 31070298, 30870180), the Zhejiang Scientific and Technological Program (2008C12081), the Hangzhou Scientific and Technological Program (20101032B25), and the Zhejiang Provincial Natural Science Foundation of China (Q13H280036).

Author information

Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310018, China

    Shang-Guo Feng, Jiang-Jie Lu, Ling Gao, Jun-Jun Liu & Hui-Zhong Wang

Authors
  1. Shang-Guo Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiang-Jie Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ling Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jun-Jun Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hui-Zhong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hui-Zhong Wang.

Additional information

S.G. Feng and J.J. Lu have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Feng, SG., Lu, JJ., Gao, L. et al. Molecular Phylogeny Analysis and Species Identification of Dendrobium (Orchidaceae) in China. Biochem Genet 52, 127–136 (2014). https://doi.org/10.1007/s10528-013-9633-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-013-9633-6

Keywords

Search

Navigation