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Genetic Structure of Tree and Shrubby Species Among Anthropogenic Edges, Natural Edges, and Interior of an Atlantic Forest Fragment

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Abstract

Two species, Psychotria tenuinervis (shrub, Rubiaceae) and Guarea guidonia (tree, Meliaceae), were used as models to compare the genetic structure of tree and shrubby species among natural edges, anthropogenic edges, and a fragment interior. There were significant differences between two genetic markers. For isozymes, P. tenuinervis presented greater heterozygosity (expected and observed) and a higher percentage of polymorphic loci and median number of alleles than G. guidonia. For microsatellites, there was no difference in genetic variability between the species. Only P. tenuinervis, for isozymes, showed differences in genetic variability among the three habitats. There was no genetic structure (F ST < 0.05) among habitats in both plant species for both genetic markers. Isozymes showed great endogamy for both plant species, but not microsatellites. The forest fragmentation may have negative effects on both spatial (among edges and interior) and temporal genetic variability.

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Acknowledgments

The authors thank Aluana G. Abreu and Sónia C. S. Andrade for help in the laboratory and Jim Hesson for correcting the English. This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant no. 150300/2004-2), and research aid no. 2001/11225-6 from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).

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Authors and Affiliations

  1. Departamento de Ciências Biológicas e da Terra, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 714 Centro, 37130-000, Alfenas, MG, Brazil

    Flavio Nunes Ramos

  2. Centro de Pesquisa e Desenvolvimento em Recursos Genéticos Vegetais, IAC, CP 28, Campinas, 13075-630, SP, Brazil

    Paula Feliciano de Lima, Maria Imaculada Zucchi & Carlos Augusto Colombo

  3. Departamento de Genética e Evolução, Universidade Estadual de Campinas, Campinas, 13083-970, Brazil

    Vera Nisaka Solferini

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  1. Flavio Nunes Ramos
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  2. Paula Feliciano de Lima
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  3. Maria Imaculada Zucchi
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Correspondence to Flavio Nunes Ramos.

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Ramos, F.N., de Lima, P.F., Zucchi, M.I. et al. Genetic Structure of Tree and Shrubby Species Among Anthropogenic Edges, Natural Edges, and Interior of an Atlantic Forest Fragment. Biochem Genet 48, 215–228 (2010). https://doi.org/10.1007/s10528-009-9311-x

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