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Mating Systems of Psychotria tenuinervis (Rubiaceae): Distance from Anthropogenic and Natural Edges of Atlantic Forest Fragment

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Abstract

The aim of this study was to determine and compare the mating systems among Psychotria tenuinervis populations at anthropogenic edges, natural edges, and the forest interior using allozyme electrophoresis of naturally pollinated progeny arrays. P. tenuinervis showed low outcrossing rates, varying from 37% to 50% of the mating attributable to outcrossing and 50% to 63% attributable to self-fertilization, in the three habitats. The forest interior had the highest outcrossing rate (t m = 0.50 and t s = 0.43) among the three habitats. However, there were no differences in either multilocus or single-locus rates among the three habitats, indicating that the contribution of biparental inbreeding to the apparent selfing rate in these populations was very low. The multilocus (t m) and single-locus (t s) outcrossing rates for the P. tenuinervis in the sample plots within each habitat showed great heterogeneity. In conclusion, edge creation seems not to influence its mating systems. Additionally, although P. tenuinervis is a distylous species, the population’s inbreeding can be attributed almost entirely to self-fertilization.

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Acknowledgments

The authors thank Vanessa Rosseto, Maíra T. Ribeiro, and Carolina B. Virillo for help with the field work, and Aluana G. Abreu and Sónia C. S. Andrade for help in the laboratory. We also thank Aluana G. Abreu, Fabio R. Scarano, Flavia F. Jesus, and Keith S. Brown Jr. for valuable comments on the manuscript. This work was supported by grant 141569/2000-0 from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and research aid 2001/11225-6 from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Proap-Capes. F. A. M. Santos was supported by a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant 207132/2004-8).

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ecologia, IB, UNICAMP, CP 6109, 13083-970, Campinas, SP, Brazil

    Flavio Nunes Ramos

  2. Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, CP 83, CEP 13400-970, Piracicaba, SP, Brazil

    Maria Imaculada Zucchi

  3. Departamento de Genética e Evolução, IB, UNICAMP, CP 6109, 13083-970, Campinas, SP, Brazil

    Vera Nisaka Solferini

  4. Departamento de Botânica, IB, UNICAMP, CP 6109, 13083-970, Campinas, SP, Brazil

    Flavio A. M. Santos

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  1. Flavio Nunes Ramos
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  4. Flavio A. M. Santos
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Correspondence to Flavio Nunes Ramos.

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Ramos, F.N., Zucchi, M.I., Solferini, V.N. et al. Mating Systems of Psychotria tenuinervis (Rubiaceae): Distance from Anthropogenic and Natural Edges of Atlantic Forest Fragment. Biochem Genet 46, 88–100 (2008). https://doi.org/10.1007/s10528-007-9132-8

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