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Lysobacter prati sp. nov., isolated from a plateau meadow sample

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Abstract

A novel proteobacterial strain designated SYSU H10001T was isolated from a soil sample collected from plateau meadow in Hongyuan county, Sichuan province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU H10001T was most closely related to Lysobacter soli KCTC 22011T (98.6%, sequence similarity) and Lysobacter panacisoli JCM 19212T (98.2%). The prediction result of secondary metabolites based on genome shown that the strain SYSU H10001T contained 3 clusters of bacteriocins, 1 cluster of non-ribosomal peptide synthetase, 1 cluster of type 1 polyketide synthase and 1 cluster of arylpolyene. In addition, the major isoprenoid quinone was Q-8 and the major fatty acids were identified as iso-C15:0, iso-C17:0 and Summed feature 9. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and three unidentified phospholipids. The genomic DNA G + C content of strain SYSU H10001T was 66.5% (genome). On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU H10001T represents a novel species of the genus Lysobacter, for which the name Lysobacter prati sp. nov. is proposed. The type strain is SYSU H10001T (= KCTC 72062T = CGMCC 1.16662T).

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Acknowledgements

The authors are grateful to Prof. Jung-Sook Lee (KCTC, Korea) and Prof. Takuji Kudo (JCM, Japan) for kindly providing the experimental control strains. This research was supported by the National Key R&D Program of China (2017YFD0200503), Fundamental research funds for the central universities, SYSU (No. 19lgpy173) and National Fundamental Fund Project Subsidy Funds of Personnel Training of China (No. J1310025). W-J Li was also supported by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Bao-Zhu Fang, Yuan-Guo Xie, Xiao-Tong Zhang, Lan Liu, Jian-Yu Jiao, Min Xiao & Wen-Jun Li

  2. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, People’s Republic of China

    Xing-Kui Zhou

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  1. Bao-Zhu Fang
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Contributions

BZF and WJL designed research and project outline. BZF, ZXK, and LL performed isolation, deposition, and identification. YGX, JYJ and XTZ performed genome analysis. BZF, MW and WJL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wen-Jun Li.

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Fang, BZ., Xie, YG., Zhou, XK. et al. Lysobacter prati sp. nov., isolated from a plateau meadow sample. Antonie van Leeuwenhoek 113, 763–772 (2020). https://doi.org/10.1007/s10482-020-01386-6

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