Abstract
A bacterial strain, designated as THG-SKA3T, was isolated from field soil of Kyung Hee University, South Korea. Cells of the isolate were observed to be Gram-negative, aerobic, rod-shaped and motile by gliding. The strain was found to grow optimally at 28 °C, at pH 7 and in absence of NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-SKA3T shared highest sequence similarity with Lysobacter niastensis KACC 11588T followed by Lysobacter panacisoli KACC 17502T, Lysobacter enzymogenes LMG 8762T and Lysobacter oryzae KCTC 22249T. The G+C content of THG-SKA3T was determined to be 68.9 mol%. The DNA–DNA relatedness values between strain THG-SKA3T and its closest phylogenetic neighbors were below 25.0 %.The major polar lipids of strain THG-SKA3T were determined to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was identified as ubiquinone 8 (Q-8). The major cellular fatty acids were identified as branched chain iso-C15:0, iso-C16:0 and unsaturated iso-C17:1 ω9c. On the basis of polyphasic data presented, it is evident that strain THG-SKA3T represents a novel species of the genus Lysobacter, for which the name Lysobacter agri sp. nov. (type strain THG-SKA3T = KACC 18283T = CSCTCC AB 2015126T) is proposed.
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Acknowledgments
This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).
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The NCBI GenBank accession number for the 16S rRNA gene sequence of strains THG-SKA3T is KM576858.
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10482_2015_510_MOESM1_ESM.tif
Supplementary Fig. S1 The maximum-likelihood tree based on 16S rRNA gene sequence analysis showing the phylogenetic relationships between strain THG-SKA3T and members of the genus Lysobacter. Bootstrap values less than 50 % were not indicated. Dyella terrae JS14-6T was used as an out group. Scale bar, 0.05 substitutions per nucleotide position. Supplementary material 1 (TIFF 109 kb)
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Supplementary Fig. S2. Transmission electron micrograph of Lysobacter agri THG-SKA3T. Bar indicated 0.5 μm. Supplementary material 2 (TIFF 1768 kb)
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Supplementary Fig. S3. Two-dimensional TLC of the total polar lipids of Lysobacter agri THG-SKA3T (a) and Lysobacter niatensis KACC 11588T (b), stained for total polar lipids with 5 % ethanolic molybdatophosphoric acid. Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; APL1-2, unidentified aminophospholipid; PL, unidentified phospholipid. Supplementary material 3 (TIFF 1609 kb)
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Singh, H., Won, K., Du, J. et al. Lysobacter agri sp. nov., a bacterium isolated from soil. Antonie van Leeuwenhoek 108, 553–561 (2015). https://doi.org/10.1007/s10482-015-0510-7
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DOI: https://doi.org/10.1007/s10482-015-0510-7