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Leucosporidium escuderoi f.a., sp. nov., a basidiomycetous yeast associated with an Antarctic marine sponge

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Abstract

A basidiomycetous yeast, strain E2A-C3-II, was isolated from a marine sponge (Hymeniacidon sp.) collected at a depth of 6 m in Fildes Bay, King George Island, Antarctica. The phylogenetic analysis revealed that the yeast isolated is related to Leucosporidium drummii, Leucosporidiella muscorum and to the Leucosporidium scottii group, including Leucosporidiella creatinivora and Leucosporidiella yakutica. The analysis of the nucleotide differences and the genetic distances of the D1/D2 domain of the LSU rDNA gene and 5.8S ITS regions support that strain E2A-C3-II represents a new species. The novel species can be distinguished from L. drummii by its ability to assimilate l-sorbose, l-rhamnose, lactose and ribitol. The maximum temperature for growth was 25 °C. On the basis of morphological, biochemical and physiological characterization, and phylogenetic and nucleotide analysis, a novel basidiomycetous yeast species, Leucosporidium escuderoi f.a., sp. nov., is proposed. The type strain is E2A-C3-IIT (=CBS 12734T =CECT 13080T). The Mycobank (http://www.mycobank.org) accession number is MB 804654. The nucleotide sequences of D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions obtained in this work have been deposited in Genbank under the Accession numbers JN181009 and JN197600, respectively.

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Acknowledgments

The authors are grateful to two anonymous reviewers for their valuable suggestions on the manuscript. F. Laich is supported by the “Recursos y Tecnologías Agrarias in coordination with the Comunidades Autónomas del Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica 2004–2007″ program, financed with the involvement of the European Social Fund. R. Chávez was supported by Instituto Antártico Chileno (INACH, Grant G_06–10) and DICYT-USACH. I. Vaca was supported by Grant Fondecyt 11090192 and PBCT PDA 13.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Instituto Canario de Investigaciones Agrarias, Ctra. Boquerón s/n. Valle de Guerra, Santa Cruz de Tenerife, Spain

    Federico Laich

  2. Departamento Central de Investigación, Universidad Laica “Eloy Alfaro” de Manabí, Av. Circunvalación y Calle 12, Manta, Ecuador

    Federico Laich

  3. Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile (USACH), Alameda 3363, 9170022, Estación Central, Santiago, Chile

    Renato Chávez

  4. Departamento de Química, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile

    Inmaculada Vaca

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  1. Federico Laich
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Correspondence to Inmaculada Vaca.

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Laich, F., Chávez, R. & Vaca, I. Leucosporidium escuderoi f.a., sp. nov., a basidiomycetous yeast associated with an Antarctic marine sponge. Antonie van Leeuwenhoek 105, 593–601 (2014). https://doi.org/10.1007/s10482-014-0114-7

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