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Cultivable psychrotolerant yeasts associated with Antarctic marine sponges

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Abstract

Unlike filamentous fungi and bacteria, very little is known about cultivable yeasts associated with marine sponges, especially those from Antarctic seas. During an expedition to King George Island, in the Antarctica, samples of 11 marine sponges were collected by scuba-diving. From these sponges, 20 psychrotolerant yeast isolates were obtained. Phylogenetic analyses of D1/D2 and ITS rRNA gene sequences revealed that the marine ascomycetous yeast Metschnikowia australis is the predominant organism associated with these invertebrates. Other species found belonged to the Basidiomycota phylum: Cystofilobasidium infirmominiatum, Rhodotorula pinicola, Leucosporidiella creatinivora and a new yeast from the Leucosporidiella genus. None of these yeasts have been previously associated with marine sponges. A screening to estimate the ability of these yeasts as producers of extracellular enzymatic activities at several pH and temperature conditions was performed. Several yeast isolates demonstrated amylolytic, proteolytic, lipolytic or cellulolytic activity, but none of them showed xylanolytic activity under the conditions assayed. To our knowledge, this work is the first description of cultivable yeasts associated with marine sponges from the Antarctic sea.

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Acknowledgments

This work was supported by Instituto Antártico Chileno (INACH) grant G_06-10, FONDECYT grant 11090192, “Programa Bicentenario de Ciencia y Tecnología” (Chile) project PDA13, and DICYT-USACH. Fermín Acosta thanks “Programa IFARHU-MEF” (República de Panamá). We thank Antarctic collaborators Dr. José Darias, Dr. María Darias, Dr. Aurelio San Martín and Carol San Martín, and all the team of Professor Julio Escudero Base in Antarctica for allowing us to use their accommodations and laboratory facilities. We also thank to Karen Vergara for lab assistance and Dr. Jaime Eyzaguirre for critical reading of this manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Departamento de Química, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile

    Inmaculada Vaca, Felipe Maza & Valentina Hernández

  2. Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Usach. Alameda 3363, Correo 33, Casilla 40, Estación Central, Santiago, Chile

    Carolina Faúndez, Braulio Paillavil, Fermín Acosta, Gloria Levicán & Renato Chávez

  3. Departamento de Ciencia y Tecnología de los Alimentos, Edificio de Alimentos, Universidad de Santiago de Chile (USACH), Obispo Umaña 50, Estación Central, Santiago, Chile

    Claudio Martínez

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  1. Inmaculada Vaca
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  2. Carolina Faúndez
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  3. Felipe Maza
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  4. Braulio Paillavil
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  5. Valentina Hernández
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  6. Fermín Acosta
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  7. Gloria Levicán
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  8. Claudio Martínez
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  9. Renato Chávez
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Correspondence to Renato Chávez.

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Vaca, I., Faúndez, C., Maza, F. et al. Cultivable psychrotolerant yeasts associated with Antarctic marine sponges. World J Microbiol Biotechnol 29, 183–189 (2013). https://doi.org/10.1007/s11274-012-1159-2

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  • DOI: https://doi.org/10.1007/s11274-012-1159-2

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