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Taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial Antarctic samples

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Abstract

The aim of the present study was to investigate the taxonomic identity of yeasts isolated from the Antarctic continent and to evaluate their ability to produce enzymes (lipase, protease and xylanase) at low and moderate temperatures. A total of 97 yeast strains were recovered from marine and terrestrial samples collected in the Antarctica. The highest amount of yeast strains was obtained from marine sediments, followed by lichens, ornithogenic soils, sea stars, Salpa sp., algae, sea urchin, sea squirt, stone with lichens, Nacella concinna, sea sponge, sea isopod and sea snail. Data from polyphasic taxonomy revealed the presence of 21 yeast species, distributed in the phylum Ascomycota (n = 8) and Basidiomycota (n = 13). Representatives of encapsulated yeasts, belonging to genera Rhodotorula and Cryptococcus were recovered from 7 different Antarctic samples. Moreover, Candida glaebosa, Cryptococcus victoriae, Meyerozyma (Pichia) guilliermondii, Rhodotorula mucilaginosa and R. laryngis were the most abundant yeast species recovered. This is the first report of the occurrence of some species of yeasts recovered from Antarctic marine invertebrates. Additionally, results from enzymes production at low/moderate temperatures revealed that the Antarctic environment contains metabolically diverse cultivable yeasts, which could be considered as a target for biotechnological applications. Among the evaluated yeasts in the present study 46.39, 37.11 and 14.43 % were able to produce lipase (at 15 °C), xylanase (at 15 °C) and protease (at 25 °C), respectively. The majority of lipolytic, proteolytic and xylanolytic strains were distributed in the phylum Basidiomycota and were mainly recovered from sea stars, lichens, sea urchin and marine sediments.

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Acknowledgments

The authors would like to thank Prof. Vivian Helena Pellizari, Prof. Itamar Soares Melo and Prof. Eduardo Carlos Hadju for their contribution in the collection of marine and terrestrial Antarctic samples, as well as Raymond J. Burgess for the English Review. A. Duarte was supported by Ph.D. grants from CAPES and FAPESP (2010/08352-5). Part of the research was supported by the European Community’s Seventh Framework Program (FP7 2010–2013, BAMMBO Project). L.D. Sette thanks FAPESP for the financial support (FAPESP grant 2010/17033-0) and PROANTAR Program for the sampling support.

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Authors and Affiliations

  1. Divisão de Recursos Microbianos (CPQBA), Universidade Estadual de Campinas, UNICAMP, Paulínia, São Paulo, Brazil

    A. W. F. Duarte, F. S. Nobre & L. D. Sette

  2. Centro de Insetos Sociais, Universidade Estadual Paulista, UNESP, Rio Claro, São Paulo, Brazil

    I. Dayo-Owoyemi

  3. Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, USP, Lorena, São Paulo, Brazil

    L. C. S. Chaud & M. G. A. Felipe

  4. Departamento de Bioquímica e Microbiologia, Universidade Estadual Paulista, UNESP, Rio Claro, São Paulo, Brazil

    F. C. Pagnocca & L. D. Sette

  5. Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, USP, São Paulo, São Paulo, Brazil

    A. Pessoa

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  1. A. W. F. Duarte
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  2. I. Dayo-Owoyemi
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Correspondence to L. D. Sette.

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Communicated by M. da Costa.

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Duarte, A.W.F., Dayo-Owoyemi, I., Nobre, F.S. et al. Taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial Antarctic samples. Extremophiles 17, 1023–1035 (2013). https://doi.org/10.1007/s00792-013-0584-y

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