Skip to main content
SpringerLink
Log in

Production of cellulosic ethanol and enzyme from waste fiber sludge using SSF, recycling of hydrolytic enzymes and yeast, and recombinant cellulase-producing Aspergillus niger

  • Bioenergy/Biofuels/Biochemicals
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Bioethanol and enzymes were produced from fiber sludges through sequential microbial cultivations. After a first simultaneous saccharification and fermentation (SSF) with yeast, the bioethanol concentrations of sulfate and sulfite fiber sludges were 45.6 and 64.7 g/L, respectively. The second SSF, which included fresh fiber sludges and recycled yeast and enzymes from the first SSF, resulted in ethanol concentrations of 38.3 g/L for sulfate fiber sludge and 24.4 g/L for sulfite fiber sludge. Aspergillus niger carrying the endoglucanase-encoding Cel7B gene of Trichoderma reesei was grown in the spent fiber sludge hydrolysates. The cellulase activities obtained with spent hydrolysates of sulfate and sulfite fiber sludges were 2,700 and 2,900 nkat/mL, respectively. The high cellulase activities produced by using stillage and the significant ethanol concentrations produced in the second SSF suggest that onsite enzyme production and recycling of enzyme are realistic concepts that warrant further attention.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Alriksson B, Rose SH, van Zyl WH, Sjöde A, Nilvebrant N-O, Jönsson LJ (2009) Cellulase production from spent lignocellulose hydrolysates by recombinant Aspergillus niger. Appl Environ Microbiol 75:2366–2374

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23:257–270

    Article  CAS  Google Scholar 

  3. Cavka A, Alriksson B, Rose SH, van Zyl WH, Jönsson LJ (2011) Biorefining of wood: combined production of ethanol and xylanase from waste fiber sludge. J Ind Microbiol Biotechnol 38:891–899

    Article  CAS  PubMed  Google Scholar 

  4. La Grange DC, den Haan R, van Zyl WH (2010) Engineering cellulolytic ability into bioprocessing organisms. Appl Microbiol Biotechnol 87:1195–1208

    Article  CAS  PubMed  Google Scholar 

  5. Image J (2014) http://rsb.info.nih.gov/ij. Accessed 21 March 2014

  6. Kang L, Wang W, Lee YY (2009) Bioconversion of kraft paper mill sludges to ethanol by SSF and SSCF. Appl Biochem Biotechnol 161:53–66

    Article  Google Scholar 

  7. Marques S, Alves L, Roseiro JC, Gýrio FM (2008) Conversion of recycled paper sludge to ethanol by SHF and SSF using Pichia stipitis. Biomass Bioenerg 32:400–406

    Article  CAS  Google Scholar 

  8. Meyer V (2008) Genetic engineering of filamentous fungi—progress, obstacles and future trends. Biotechnol Adv 26:177–185

    Article  CAS  PubMed  Google Scholar 

  9. Montenecourt BS (1983) Trichoderma reesei cellulases. Trends Biotechnol 1:156–161

    Article  CAS  Google Scholar 

  10. Park CS, Chang CC, Ryu DDY (2000) Expression and high-level secretion of Trichoderma reesei endoglucanase I in Yarrowia lipolytica. Appl Biochem Biotechnol 87:1–15

    Article  CAS  PubMed  Google Scholar 

  11. Parkinson M, Wainwright M, Killham K (1989) Observations on oligotrophic growth of fungi on silica gel. Mycol Res 93:529–534

    Article  Google Scholar 

  12. Rose SH, van Zyl WH (2002) Constitutive expression of the Trichoderma reesei beta-1,4-xylanase gene (xyn2) and the beta-1,4-endoglucanase gene (egI) in Aspergillus niger in molasses and defined glucose media. Appl Microbiol Biotechnol 58:461–468

    Article  CAS  PubMed  Google Scholar 

  13. Sjöde A, Alriksson B, Jönsson LJ, Nilvebrant N-O (2007) The potential in bioethanol production from waste fiber sludges in pulp mill-based biorefineries. Appl Biochem Biotechnol 136–140:327–338

    Google Scholar 

  14. Stals I, Sandra K, Geysens S, Contreras R, Van Beeumen J, Claeyssens M (2004) Factors influencing glycosylation of Trichoderma reesei cellulases. I: postsecretorial changes of the O- and N-glycosylation pattern of Cel7A. Glycobiology 14:713–724

    Article  CAS  PubMed  Google Scholar 

  15. Steinberg TH, Haugland RP, Singer VL (1996) Applications of SYPRO Orange and SYPRO Red protein gel stains. Anal Biochem 239:238–245

    Article  CAS  PubMed  Google Scholar 

  16. Takashima S, Nakamura A, Hidaka M, Masaki H, Uozumi T (1999) Isolation and characterization of the actin gene from the cellulolytic fungus Humicola grisea and analysis of transcription levels of actin and cellulase genes. J Biochem 125:728–736

    Article  CAS  PubMed  Google Scholar 

  17. Wainwright M, Al-Wajee K, Grayston SJ (1997) Effect of silicic acid and other silicon compounds on fungal growth in oligotrophic and nutrient-rich media. Mycol Res 101:933–938

    Article  CAS  Google Scholar 

  18. Webb L (1994) Sludge disposal: the unregulated loophole. Pulp Paper 36:18–23

    Google Scholar 

  19. Wingren A, Galbe M, Zacchi G (2003) Techno-economic evaluation of producing ethanol from softwood: comparison of SSF and SHF and identification of bottlenecks. Biotechnol Prog 19:1109–1117

    Article  CAS  PubMed  Google Scholar 

  20. Yamashita Y, Kurosumi A, Sasaki C, Nakamura Y (2008) Ethanol production from paper sludge by immobilized Zymomonas mobilis. Biochem Eng J 42:314–319

    Article  CAS  Google Scholar 

  21. Zhang CQ, Qi W, Wang F, Li Q, Su RX, He ZM (2011) Ethanol from corn stover using SSF: an economic assessment. Energy Sources Part B 6:136–144

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank the pulp mills for providing the fiber sludge. One of the authors (AC) was supported by SEKAB E-Technology AB (Örnsköldsvik, Sweden) through the Umeå University Industrial Graduate School. Mass spectrometry was performed by Dr. T. Kieselbach at the KBC Proteomics Core Facility of Umeå University and the Swedish University of Agricultural Sciences in Umeå. The research received financial support from The Kempe Foundations, The Knut and Alice Wallenberg Foundation, The Swedish Energy Agency, and Bio4Energy (http://www.bio4energy.se).

Author information

Authors and Affiliations

  1. Department of Chemistry, Umeå University, 901 87, Umeå, Sweden

    Adnan Cavka & Leif J. Jönsson

  2. SP Processum AB, 891 22, Örnsköldsvik, Sweden

    Björn Alriksson

  3. Department of Microbiology, Stellenbosch University, Stellenbosch, 7602, South Africa

    Shaunita H. Rose & Willem H. van Zyl

Authors
  1. Adnan Cavka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Björn Alriksson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaunita H. Rose
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Willem H. van Zyl
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Leif J. Jönsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adnan Cavka.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cavka, A., Alriksson, B., Rose, S.H. et al. Production of cellulosic ethanol and enzyme from waste fiber sludge using SSF, recycling of hydrolytic enzymes and yeast, and recombinant cellulase-producing Aspergillus niger . J Ind Microbiol Biotechnol 41, 1191–1200 (2014). https://doi.org/10.1007/s10295-014-1457-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-014-1457-9

Keywords

Search

Navigation