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High-cell-density culture strategies for polyhydroxyalkanoate production: a review

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Journal of Industrial Microbiology & Biotechnology

Abstract

This article gives an overview of high-cell-density cultures for polyhydroxyalkanoate (PHA) production and their modes of operation for increasing productivity. High cell densities are very important in PHA production mainly because this polymer is an intracellular product accumulated in various microorganisms, so a high cellular content is needed for the polymer production. This review describes relevant results from fed-batch, repeated batch, and continuous modes of operation without and with cell recycle for the production of these polymers by microorganisms. Finally, recombinant microorganisms for PHA production, as well future directions for PHA production, are discussed.

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Abbreviations

(3HV):

3-Hydroxyvalerate

AP:

Analytical purity

(C/PA):

Carbon source/propionic acid ratio

C/N:

Carbon source sucrose or sugar cane molasses/nitrogen source urea or ammonium sulfate

DO:

Dissolved oxygen

FOS:

Fructooligosaccharides

GBL:

γ-Butyrolactone

GRP:

Waste glycerol

HCDC:

High-cell-density cultures

MM:

Mineral medium

P(3HB):

Poly(3-hydroxybutyrate)

P(3HB-4HB):

Poly(3-hydroxybutyrate–4-hydroxybutyrate)

P(3HB-4HB-3HV):

Poly(3-hydroxybutyrate–4-hydroxybutyrate–3-hydroxyvalerate)

P(3HB-co-3HV):

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

P(3HB-co-4HB):

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)

%PHAs:

Polyhydroxyalkanoate cell content

PA:

Propionic acid

PHA:

Polyhydroxyalkanoates

PHASCL :

Short-chain-length polyhydroxyalkanoates

PHASMCL :

Medium-chain-length polyhydroxyalkanoates

r PHAS :

Productivity of polyhydroxyalkanoates

STR:

Stirred tank reactors

Xr:

Residual biomass

Xt:

Total biomass concentration

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  1. Brazilian Bioethanol Science and Technology Laboratory, CTBE/CNPEM, P.O. Box 6070, Campinas, SP, CEP 13083-970, Brazil

    Jaciane Lutz Ienczak

  2. Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), P.O. Box 476, Florianópolis, SC, CEP 88040-900, Brazil

    Willibaldo Schmidell & Gláucia Maria Falcão de Aragão

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Ienczak, J.L., Schmidell, W. & de Aragão, G.M.F. High-cell-density culture strategies for polyhydroxyalkanoate production: a review. J Ind Microbiol Biotechnol 40, 275–286 (2013). https://doi.org/10.1007/s10295-013-1236-z

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