Abstract
Batch cultivation of Ralstonia eutropha NRRL B14690 attained 21 g biomass l−1 and 9.4 g poly(β-hydroxybutyrate) l−1 (0.45 g PHB g−1 dry wt−1) in 60 h. Repeated batch operation (empty-and-fill protocol) to remove 20% (v/v) of the culture broth and to supplement an equal volume of fresh media resulted in 49 g biomass l−1 and 25 g PHB l−1 (0.51 g PHB g−1 dry wt−1) with an overall productivity of 0.42 g PHB l−1 h−1 in 67 h. In the two cycles of repeated batch fermentation there was a 3-fold increase in productivity as compared to batch.
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Khanna, S., Srivastava, A.K. Repeated Batch Cultivation of Ralstonia eutropha for Poly (β-hydroxybutyrate) Production. Biotechnol Lett 27, 1401–1403 (2005). https://doi.org/10.1007/s10529-005-0689-1
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DOI: https://doi.org/10.1007/s10529-005-0689-1