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A thermostable phytase from Bacillus sp. MD2: cloning, expression and high-level production in Escherichia coli

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Phytase is used as a feed additive for degradation of antinutritional phytate, and the enzyme is desired to be highly thermostable for it to withstand feed formulation conditions. A Bacillus sp. MD2 showing phytase activity was isolated, and the phytase encoding gene was cloned and expressed in Escherichia coli. The recombinant phytase exhibited high stability at temperatures up to 100°C. A higher enzyme activity was obtained when the gene expression was done in the presence of calcium chloride. Production of the enzyme by batch- and fed-batch cultivation in a bioreactor was studied. In batch cultivation, maintaining dissolved oxygen at 20–30% saturation and depleting inorganic phosphate below 1 mM prior to induction by IPTG resulted in over 10 U/ml phytase activity. For fed–batch cultivation, glucose concentration was maintained at 2–3 g/l, and the phytase expression was increased to 327 U/ml. Induction using lactose during fed-batch cultivation showed a lag phase of 4 h prior to an increase in the phytase activity to 71 U/ml during the same period as IPTG-induced production. Up to 90% of the total amount of expressed phytase leaked out from the E. coli cells in both IPTG- and lactose-induced fed-batch cultivations.

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Acknowledgment

The financial support of the Swedish Agency for Development Cooperation (Sida/SAREC) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Lund University, Box 124, 221 00, Lund, Sweden

    Thi Thuy Tran, Gashaw Mamo, Bo Mattiasson & Rajni Hatti-Kaul

  2. Biotechnology and Microbiology Department, Hanoi National University of Education, 136 Xuan Thuy Street, Hanoi, Vietnam

    Thi Thuy Tran

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  1. Thi Thuy Tran
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  2. Gashaw Mamo
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Correspondence to Rajni Hatti-Kaul.

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Tran, T.T., Mamo, G., Mattiasson, B. et al. A thermostable phytase from Bacillus sp. MD2: cloning, expression and high-level production in Escherichia coli . J Ind Microbiol Biotechnol 37, 279–287 (2010). https://doi.org/10.1007/s10295-009-0671-3

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