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VrDREB2A, a DREB-binding transcription factor from Vigna radiata, increased drought and high-salt tolerance in transgenic Arabidopsis thaliana

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Abstract

Mung bean (Vigna radiata L.) is commonly grown in Asia as an important nutritional dry grain legume, as it can survive better in arid conditions than other crops. Abiotic stresses, such as drought and high-salt contents, negatively impact its growth and production. The dehydration-responsive element-binding protein 2 (DREB2) transcription factors play a significant role in the response to these stress stimuli via transcriptional regulation of downstream genes containing the cis-element dehydration-responsive element (DRE). However, the molecular mechanisms involved in the drought tolerance of this species remain elusive, with very few reported candidate genes. No DREB2 ortholog has been reported for mung bean, and the function of mung bean DREB2 is not clear. In this study, a novel VrDREB2A gene with conserved AP2 domains and transactivation ability was isolated from mung bean. A modified VrDREB2A protein lacking the putative negative regulatory domain encoded by nucleotides 394–543 was shown to be localized in the nucleus. Expression of the VrDREB2A gene was induced by drought, high salt concentrations and abscisic acid treatment. Furthermore, comparing with the wild type Arabidopsis, the overexpression of VrDREB2A activated the expression of downstream genes in transgenic Arabidopsis, resulting in enhanced tolerance to drought and high-salt stresses and no growth retardation. The results from this study indicate that VrDREB2A functions as an important transcriptional activator and may help increase the abiotic stress tolerance of the mung bean plant.

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Acknowledgments

This work was supported by the Ministry of Agriculture of China [the earmarked fund for China Agriculture Research System (CARS-09)] and the Agricultural Science and Technology Innovation Program (ASTIP) of CAAS.

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Authors and Affiliations

  1. Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture; National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Honglin Chen, Lixia Wang, Suhua Wang & Xuzhen Cheng

  2. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Liping Liu

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  1. Honglin Chen
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  2. Liping Liu
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  3. Lixia Wang
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  4. Suhua Wang
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  5. Xuzhen Cheng
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Correspondence to Xuzhen Cheng.

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10265_2015_773_MOESM1_ESM.pdf

Additional Supporting Information may be found in the online version of this article: Table S1 Sequence of oligonucleotides used for cloning and sequencing analyses of VrDREB2A (CDS), Localization and transactivation ability of VrDREB2A and qRT-PCR of transgenic Arabidopsis. Table S2 Primers used for qRT-PCR expression analysis of genes downstream of VrDREB2A in Arabidopsis (PDF 117 kb)

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Chen, H., Liu, L., Wang, L. et al. VrDREB2A, a DREB-binding transcription factor from Vigna radiata, increased drought and high-salt tolerance in transgenic Arabidopsis thaliana . J Plant Res 129, 263–273 (2016). https://doi.org/10.1007/s10265-015-0773-0

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  • DOI: https://doi.org/10.1007/s10265-015-0773-0

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