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GmPGIP3 enhanced resistance to both take-all and common root rot diseases in transgenic wheat

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Abstract

Take-all (caused by the fungal pathogen Gaeumannomyces graminis var. tritici, Ggt) and common root rot (caused by Bipolaris sorokiniana) are devastating root diseases of wheat (Triticum aestivum L.). Development of resistant wheat cultivars has been a challenge since no resistant wheat accession is available. GmPGIP3, one member of polygalacturonase-inhibiting protein (PGIP) family in soybean (Glycine max), exhibited inhibition activity against fungal endopolygalacturonases (PGs) in vitro. In this study, the GmPGIP3 transgenic wheat plants were generated and used to assess the effectiveness of GmPGIP3 in protecting wheat from the infection of Ggt and B. sorokiniana. Four independent transgenic lines were identified by genomic PCR, Southern blot, and reverse transcription PCR (RT-PCR). The introduced GmPGIP3 was integrated into the genomes of these transgenic lines and could be expressed. The expressing GmPGIP3 protein in these transgenic wheat lines could inhibit the PGs produced by Ggt and B. sorokiniana. The disease response assessments postinoculation showed that the GmPGIP3-expressing transgenic wheat lines displayed significantly enhanced resistance to both take-all and common root rot diseases caused by the infection of Ggt and B. sorokiniana. These data suggested that GmPGIP3 is an attractive gene resource in improving resistance to both take-all and common root rot diseases in wheat.

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Acknowledgments

This study was supported by the National “Key Sci-Tech” program, China (Grant no.2013ZX08002001-004).

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Authors and Affiliations

  1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, China

    Aiyun Wang & Wei Rong

  2. The National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetic and Breeding of Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Aiyun Wang, Xuening Wei, Wei Rong, Liang Dang, Li-Pu Du, Lin Qi, Hui-Jun Xu & Zengyan Zhang

  3. College of Biology Science, Agricultural University of Hebei, Baoding, 071001, China

    Liang Dang & Yanjun Shao

Authors
  1. Aiyun Wang
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  2. Xuening Wei
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  9. Zengyan Zhang
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Correspondence to Zengyan Zhang.

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Aiyun Wang and Xuening Wei contributed equally to this work.

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Wang, A., Wei, X., Rong, W. et al. GmPGIP3 enhanced resistance to both take-all and common root rot diseases in transgenic wheat. Funct Integr Genomics 15, 375–381 (2015). https://doi.org/10.1007/s10142-014-0428-6

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  • DOI: https://doi.org/10.1007/s10142-014-0428-6

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