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PtDRG1, a Desiccation Response Gene from Pyropia tenera (Rhodophyta), Exhibits Chaperone Function and Enhances Abiotic Stress Tolerance

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Abstract

Pyropia are commercially valuable marine red algae that grow in the intertidal zone. They are extremely tolerant to desiccation stress. We have previously identified and reported desiccation response genes (DRGs) based on transcriptome analysis of P. tenera. Among them, PtDRG1 encodes a polypeptide of 22.6 kDa that is located in the chloroplast. PtDRG1 does not share sequence homology with any known gene deposited in public database. Transcription of PtDRG1 gene was upregulated by osmotic stress induced by mannitol or H2O2 as well as desiccation stress, but not by heat. When PtDRG1 was overexpressed in Escherichia coli or Chlamydomonas, transformed cells grew much better than control cells under high temperature as well as osmotic stress induced by mannitol and NaCl. In addition, PtDRG1 significantly reduced thermal aggregation of substrate protein under heat stress condition. These results demonstrate that PtDRG1 has a chaperone function and plays a role in tolerance mechanism for abiotic stress. This study shows that red algae have unknown stress proteins such as PtDRG1 that contributes to stress tolerance.

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Funding

This work was supported by Korean Institute of Planning and Evaluation for Technology, Agriculture, Forestry and Fisheries (IPET) through Golden Seed Project (Project number, 213008-05-2-SB830) funded by the Ministry of Oceans and Fisheries (MOF), Republic of Korea.

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Authors and Affiliations

  1. Department of Biology Education, Chonnam National University and Khumho Research Institute, Gwangju, 61186, South Korea

    Yeonju Na, Ha-Nul Lee, Jiwoong Wi & Dong-Woog Choi

  2. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, South Korea

    Won-Joong Jeong

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  1. Yeonju Na
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  2. Ha-Nul Lee
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  3. Jiwoong Wi
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  4. Won-Joong Jeong
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Correspondence to Dong-Woog Choi.

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Electronic Supplementary Material

Supplementary Fig. 1

Chaperone activity of PtDRG1 at various concentrations. Chaperone function of PtDRG1 was examined using alcohol dehydrogenase (ADH) as a substrate. To induce aggregation, ADH was heated to 55 °C. Thermal aggregation of ADH was determined by monitoring the turbidity at A360 for 50 min in the presence of various concentrations of PtDRG1 protein (0.1–5 mg/ml). Standard deviations (vertical bar) were calculated from data obtained from three replicate experiments. (PPTX 64 kb)

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Na, Y., Lee, HN., Wi, J. et al. PtDRG1, a Desiccation Response Gene from Pyropia tenera (Rhodophyta), Exhibits Chaperone Function and Enhances Abiotic Stress Tolerance. Mar Biotechnol 20, 584–593 (2018). https://doi.org/10.1007/s10126-018-9828-2

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