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Hydrogen peroxide released from Pyropia yezoensis induced by oligo-porphyrans: Mechanisms and effect

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Abstract

Porphyran, a sulfated galactan, is the main cellular polysaccharide in the marine red alga Pyropia yezoensis. In this study, oligo-porphyrans, obtained by acid hydrolysis of porphyran, were investigated for their H2O2-inducing abilities in the defense responses of P. yezoensis. Oligo-porphyrans with average molecular weights (MWs) lower than 1.43 kDa had H2O2-inducing abilities. In contrast, oligo-porphyrans with average MWs of 6.12 kDa triggered no response. The active oligo-porphyrans were fractioned by anion-exchange chromatography. We found that two distinct mechanisms might be involved in the oligo-porphyran-induced H2O2 release in P. yezoensis. Mixtures of mono-sulfated oligo-galactans with degrees of polymerization (DPs) ranging from 1 to 3 might induce the response through the oxidation of cellular oligosaccharides, which enable P. yezoensis to resist rotting caused by dense incubation. Mixtures of oligo-porphyrans, consisting of 4 ~ 7 monosaccharide residues and 2 ~ 3 sulfate groups, might induce the generation of H2O2 by activation of NADPH oxidase, leading to an oxidative burst in P. yezoensis. The elicitor activity of oligo-porphyrans thus depends on their molecular size.

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Acknowledgments

This work was supported by the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-QN204); National Key Technology Research & Development Program (2013BAB01B01); Special Fund for Marine Scientific Research in the Public Interest (201405040); Shandong Province Science and Technology Development Project (2014GHY115017); and Applied Research Projects of Nantong Municipal (BK2013011). The authors thank Prof. Zhien Li and Zuhong Xu (Institute of Oceanology, Chinese Academy of Sciences) for their helpful assistance in the experiments.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, 7 Naihai Road, Qingdao, 266071, People’s Republic of China

    Yun Hou, Jing Wang, Weihua Jin, Hong Zhang & Quanbin Zhang

  2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Yun Hou

  3. Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Nantong, Jiangsu, 226006, People’s Republic of China

    Jing Wang & Quanbin Zhang

  4. Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA

    Thomas Simerly

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  1. Yun Hou
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  2. Jing Wang
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  3. Thomas Simerly
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  4. Weihua Jin
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  5. Hong Zhang
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Correspondence to Quanbin Zhang.

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Hou, Y., Wang, J., Simerly, T. et al. Hydrogen peroxide released from Pyropia yezoensis induced by oligo-porphyrans: Mechanisms and effect. J Appl Phycol 27, 1639–1649 (2015). https://doi.org/10.1007/s10811-014-0471-7

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  • DOI: https://doi.org/10.1007/s10811-014-0471-7

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